The metabotropic glutamate receptor subtype mGluR 2/3 is located at extrasynaptic loci in rat spinal dorsal horn synapses

被引：31

作者：

Azkue, JJ ^{[1
]}

Mateos, JM ^{[1
]}

Elezgarai, I ^{[1
]}

Benítez, R ^{[1
]}

Osorio, A ^{[1
]}

Díez, J ^{[1
]}

Bilbao, A ^{[1
]}

Bidaurrazaga, A ^{[1
]}

Grandes, P ^{[1
]}

机构：

[1] Univ Basque Country, Sch Med & Dent, Dept Neurosci, Bilbao, Spain

来源：

NEUROSCIENCE LETTERS | 2000年 / 287卷 / 03期

关键词：

immunocytochemistry; spinal cord; excitatory amino acids;

D O I：

10.1016/S0304-3940(00)01189-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The position of neurotransmitter receptors relative to active neurotransmitter release sites may be a major factor influencing neuronal responses. The location of the metabotropic glutamate receptor subtype mGluR2/3 was investigated in synaptic structures in the rat superficial spinal dorsal horn laminae by using a pre-embedding immunogold technique. Immunostaining for mGluR2/3 occurred in laminae I through III. Gold particles were encountered both in the cytosol and along the plasma membrane. Distinctive plasmalemmal immunodeposits were detected in vesicle-containing profiles, where they were located to membrane compartments distant from active release sites rather than in the close vicinity of synaptic specialisations. No distinct immunolabelling was observed in profiles meeting characteristics of primary afferent terminals. The extrasynaptic occurrence of mGluR2/3 suggests a presynaptic heteroreceptor role for these receptor subtypes in the spinal dorsal horn. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：236 / 238

页数：3

共 20 条

[1]

BAUDE A, 1994, J NEUROSCI, V14, P2830

[2] HIGH-RESOLUTION IMMUNOGOLD LOCALIZATION OF AMPA TYPE GLUTAMATE-RECEPTOR SUBUNITS AT SYNAPTIC AND NONSYNAPTIC SITES IN RAT HIPPOCAMPUS [J].