Hyperspectral imaging acousto-optic system with spatial filtering for optical phase visualization

被引:24
作者
Yushkov, Konstantin B. [1 ]
Molchanov, Vladimir Ya. [1 ]
机构
[1] Natl Univ Sci & Technol MISIS, Acoustoopt Res Ctr, Moscow, Russia
基金
俄罗斯基础研究基金会;
关键词
acousto-optics; hyperspectral imaging; spatial filtering; microscopy; GRADIENT FIELD MICROSCOPY; IDENTIFICATION; CELLS;
D O I
10.1117/1.JBO.22.6.066017
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We report a method for phase visualization in the images of transparent specimens using analog image processing in incoherent light. The experimental technique is based on adaptive bandpass spatial filtering with an amplitude mask matched with an acousto-optic tunable filter in a telecentric optical system. We demonstrate the processing of microscopic images of unstained and stained histological sections of human thyroid tumor with improved contrast. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
引用
收藏
页数:7
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