Proteome Analysis of Cytoplasmatic and Plastidic β-Carotene Lipid Droplets in Dunaliella bardawil

被引:79
作者
Davidi, Lital [1 ]
Levin, Yishai [2 ]
Ben-Dor, Shifra [3 ]
Pick, Uri [1 ]
机构
[1] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel
[2] Weizmann Inst Sci, Nancy & Stephen Grand Israel Natl Ctr Personalize, IL-76100 Rehovot, Israel
[3] Weizmann Inst Sci, Biol Serv Unit, IL-76100 Rehovot, Israel
关键词
CUTICULAR WATER PERMEABILITY; VESICLE-INDUCING PROTEIN; EPICUTICULAR WAXES; CHLAMYDOMONAS-REINHARDTII; CHEMICAL-COMPOSITION; FUNCTIONAL GENOMICS; ECERIFERUM MUTANTS; SECONDARY ALCOHOLS; WEB SERVER; ARABIDOPSIS;
D O I
10.1104/pp.114.248450
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The halotolerant green alga Dunaliella bardawil is unique in that it accumulates under stress two types of lipid droplets: cytoplasmatic lipid droplets (CLD) and beta-carotene-rich (beta C) plastoglobuli. Recently, we isolated and analyzed the lipid and pigment compositions of these lipid droplets. Here, we describe their proteome analysis. A contamination filter and an enrichment filter were utilized to define core proteins. A proteome database of Dunaliella salina/D. bardawil was constructed to aid the identification of lipid droplet proteins. A total of 124 and 42 core proteins were identified in beta C-plastoglobuli and CLD, respectively, with only eight common proteins. Dunaliella spp. CLD resemble cytoplasmic droplets from Chlamydomonas reinhardtii and contain major lipid droplet-associated protein and enzymes involved in lipid and sterol metabolism. The beta C-plastoglobuli proteome resembles the C. reinhardtii eyespot and Arabidopsis (Arabidopsis thaliana) plastoglobule proteomes and contains carotene-globule-associated protein, plastid-lipid-associated protein-fibrillins, SOUL heme-binding proteins, phytyl ester synthases, beta-carotene biosynthesis enzymes, and proteins involved in membrane remodeling/lipid droplet biogenesis: VESICLE-INDUCING PLASTID PROTEIN1, synaptotagmin, and the eyespot assembly proteins EYE3 and SOUL3. Based on these and previous results, we propose models for the biogenesis of beta C-plastoglobuli and the biosynthesis of beta -carotene within beta C-plastoglobuli and hypothesize that beta C-plastoglobuli evolved from eyespot lipid droplets.
引用
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页码:60 / +
页数:30
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