Isolation and Characterisation of collagen from the skin of brownbanded bamboo shark (Chiloscyllium punctatum)

被引：145

作者：

Kittiphattanabawon, Phanat ^{[1
]}

Benjakul, Soottawat ^{[1
]}

Visessanguan, Wonnop ^{[2
]}

Kishimura, Hideki ^{[3
]}

Shahidi, Fereidoon ^{[4
]}

机构：

[1] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai 90112, Songkhla, Thailand

[2] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Klongluang 12120, Pathumthani, Thailand

[3] Hokkaido Univ, Res Fac Fisheries Sci, Lab Marine Prod & Food Sci, Hakodate, Hokkaido 0418611, Japan

[4] Mem Univ Newfoundland, Dept Biochem, St John, NF A1B 3X9, Canada

来源：

FOOD CHEMISTRY | 2010年 / 119卷 / 04期

关键词：

Collagen; Shark; Skin; ASC; PSC; Brownbanded bamboo shark; Chiloscyllium punctatum; Zeta potential; ACID-SOLUBLE COLLAGEN; SPECTROSCOPY; PEPSIN; CONFORMATION; EXTRACTION; PEPTIDES; BONE;

D O I：

10.1016/j.foodchem.2009.09.037

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the skin of brownbanded bamboo shark (Chiloscyllium punctatum) were isolated and characterised. The yield of ASC and PSC were 9.38% and 8.86% (wet weight basis), respectively. Based on protein patterns and TOYOPEARL(R) CM-650M column chromatography, both collagens contained alpha- and beta-chains as their major components. These were characterised as type I collagen with the cross-link of alpha 2-chain. As digested by V8-protease and lysyl endopeptidase, peptide maps of both ASC and PSC were similar, but differed from that of type I collagen from calf skin. Fourier transform infrared (FTIR) spectra of both collagens were similar and pepsin hydrolysis had no effect on triple-helical structure of collagen. Transition temperature (T-max) of ASC and PSC were 34.45 and 34.52 degrees C, respectively, as determined by differential scanning colorimetry (DSC). From zeta potential study, the isoelectric points of ASC and PSC were estimated to be 6.21 and 6.56, respectively. Therefore, the skin of brownbanded bamboo shark could serve as an alternative source of collagen for different applications. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1519 / 1526

页数：8

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