Protecting Enzymes from Stress-Induced Inactivation

被引:47
作者
Piszkiewicz, Samantha [1 ]
Pielak, Gary J. [1 ,2 ,3 ,4 ]
机构
[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA
[3] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA
[4] Univ N Carolina, Integrat Program Biol & Genome Sci, Chapel Hill, NC 27599 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
INTRINSICALLY DISORDERED PROTEINS; VACUUM-DRIED GLASSES; LACTATE-DEHYDROGENASE; TREHALOSE TRANSPORTER; DESICCATION TOLERANCE; ARABIDOPSIS-THALIANA; FREEZE DENATURATION; COMPATIBLE SOLUTES; LEA PROTEINS; STABILIZATION;
D O I
10.1021/acs.biochem.9b00675
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pharmaceutical and chemical industries depend on additives to protect enzymes and other proteins against stresses that accompany their manufacture, transport, and storage. Common stresses include vacuum-drying, freeze-thawing, and freeze-drying. The additives include sugars, compatible osmolytes, amino acids, synthetic polymers, and both globular and disordered proteins. Scores of studies have been published on protection, but the data have never been analyzed systematically. To spur efforts to understand the sources of protection and ultimately develop more effective formulations, we review ideas about the mechanisms of protection, survey the literature searching for patterns of protection, and then compare the ideas to the data.
引用
收藏
页码:3825 / 3833
页数:9
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