Triplex molecular beacons as modular probes for DNA detection

被引：122
作者：
Grossmann, Tom N. [1 ]
Roeglin, Lars [1 ]
Seitz, Oliver [1 ]
机构：
[1] Humboldt Univ, Inst Chem, D-12489 Berlin, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 27期
关键词：
bioanalysis; DNA recognition; fluorescent probes; FRET (fluorescence resonant energy transfer); molecular beacons;
D O I：
10.1002/anie.200700289
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Reporting live: Triplex formation is used to construct a stem-loop probe, which is opened upon binding of the DNA target (red). The triplex molecular beacon is composed of a single DNA strand (blue), a stem-forming oligomer (black), and is labeled with a fluorophore and a quencher (red and blue circles). This concept facilitates the introduction of further functionalities such as additional quenchers in the assembly of superquenched" beacons. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA."
引用
收藏
页码：5223 / 5225
页数：3
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