Triplex molecular beacons as modular probes for DNA detection

被引：124

作者：

Grossmann, Tom N. ^{[1
]}

Roeglin, Lars ^{[1
]}

Seitz, Oliver ^{[1
]}

机构：

[1] Humboldt Univ, Inst Chem, D-12489 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 27期

关键词：

bioanalysis; DNA recognition; fluorescent probes; FRET (fluorescence resonant energy transfer); molecular beacons;

D O I：

10.1002/anie.200700289

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Reporting live: Triplex formation is used to construct a stem-loop probe, which is opened upon binding of the DNA target (red). The triplex molecular beacon is composed of a single DNA strand (blue), a stem-forming oligomer (black), and is labeled with a fluorophore and a quencher (red and blue circles). This concept facilitates the introduction of further functionalities such as additional quenchers in the assembly of superquenched" beacons. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA."

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收藏

页码：5223 / 5225

页数：3

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