Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)

被引：6

作者：

Kittler, Stefan ^{[1
]}

Slouka, Christoph ^{[1
]}

Pell, Andreas ^{[1
]}

Lamplot, Roman ^{[1
]}

Besleaga, Mihail ^{[1
]}

Ablasser, Sarah ^{[1
]}

Herwig, Christoph ^{[2
]}

Spadiut, Oliver ^{[1
]}

Kopp, Julian ^{[1
]}

机构：

[1] TU Wien, Inst Chem Environm & Biosci Engn, Res Div Integrated Bioproc Dev, Gumpendorfer Str 1a, A-1060 Vienna, Austria

[2] TU Wien, Inst Chem Environm & Biosci Engn, Getreidemarkt 9-166, A-1060 Vienna, Austria

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

RECOMBINANT PROTEIN-PRODUCTION; ESCHERICHIA-COLI; FED-BATCH; EXPRESSION; LACTOSE; INDUCTION; QUALITY; GLUCOSE; SYSTEM; GROWTH;

D O I：

10.1038/s41598-021-90899-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant protein expression can be spatially separated. This cultivation mode was found to facilitate stable protein expression using microbial hosts, however mechanistic knowledge of this cultivation strategy is scarce. In this contribution we present a method workflow to reduce workload and accelerate the establishment of stable continuous processes with E. coli BL21(DE3) exclusively based on bioengineering methods.

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页数：11

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