A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from cicada (Cicada flammata)

被引：5
作者：
Ye, Xiu Juan [1 ]
Ng, Tzi Bun [1 ]
机构：
[1] Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2010年 / 86卷 / 05期
关键词：
Cicada; Lectin; Antiproliferative; HIV-1 reverse transcriptase; CARBOHYDRATE-BINDING SPECIFICITY; ACID-SPECIFIC LECTIN; C-TYPE LECTIN; PHYSICOCHEMICAL CHARACTERIZATION; BIOLOGICAL-PROPERTIES; SARCOPHAGA-PEREGRINA; CDNA CLONING; PURIFICATION; HEMOLYMPH; PROTEIN;
D O I：
10.1007/s00253-009-2363-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A dimeric lectin with a molecular weight of 60 kDa and high hemagglutinating activity was isolated from dried cicadas. It was adsorbed on Q-Sepharose and unadsorbed on Affi-Gel Blue gel. Its hemagglutinating activity was stable up to 55 A degrees C and between pH 2 and 13. The activity was inhibited by glucuronic acid and raffinose, K+ ions, and Mg2+ ions. Cicada lectin potently inhibited proliferation of HepG2 hepatoma and MCF 7 breast cancer cells, with an IC50 value of 0.76 and 0.49 A mu M, respectively. It potently inhibited HIV-1 reverse transcriptase activity with an IC50 of 0.36 mu M but was devoid of mitogenic activity on spleen cells. Its N-terminal sequence exhibited slight similarity to a conserved hypothetical protein from Culex quinquefasciatus and a gene product from transcript GH19834-RA of Drosophila grimshawi, but there was no resemblance to lectins from other insects, including Drosophila, Sarcophaga, Glossina, and Aedes species.
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页码：1409 / 1418
页数：10
相关论文
共 71 条
[1]  
AHMED H, 1989, J BIOL CHEM, V264, P18673
[2]   PURIFICATION OF A SIALIC ACID-SPECIFIC LECTIN FROM THE INDIAN SCORPION HETEROMETRUS-GRANULOMANUS [J].
AHMED, H ;
CHATTERJEE, BP ;
KELM, S ;
SCHAUER, R .
BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1986, 367 (06) :501-506
[3]   BINDING-SPECIFICITY OF A HUMAN-LEUKOCYTE CARBOHYDRATE-BINDING PROTEIN [J].
ALLEN, HJ .
IMMUNOLOGICAL INVESTIGATIONS, 1986, 15 (04) :379-392
[4]   Anti-HIV I/II activity and molecular cloning of a novel mannose/sialic acid-binding lectin from rhizome of Polygonatum cyrtonema Hua [J].
An, J ;
Liu, JZ ;
Wu, CF ;
Li, J ;
Dai, L ;
Van Damme, E ;
Balzarini, J ;
De Clercq, E ;
Chen, F ;
Bao, JK .
ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2006, 38 (02) :70-78
[5]   Marked depletion of glycosylation sites in HIV-1 gp120 under selection pressure by the mannose-specific plant lectins of Hippeastrum hybrid and Galanthus nivalis [J].
Balzarini, J ;
Van Laethem, K ;
Hatse, S ;
Froeyen, M ;
Van Damme, E ;
Bolmstedt, A ;
Peumans, W ;
De Clercq, E ;
Schols, D .
MOLECULAR PHARMACOLOGY, 2005, 67 (05) :1556-1565
[6]   Isolation and characterization of new GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus [J].
Belogortseva, NI ;
Molchanova, VI ;
Kurika, AV ;
Skobun, AS ;
Glazkova, VE .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1998, 119 (01) :45-50
[7]   HIV-1 protease inhibits its homologous reverse transcriptase by protein-protein interaction [J].
Bottcher, M ;
Grosse, F .
NUCLEIC ACIDS RESEARCH, 1997, 25 (09) :1709-1714
[8]   Isolation and characterization of Myrianthus holstii lectin, a potent HIV-1 inhibitory protein from the plant Myrianthus holstii [J].
Charan, RD ;
Munro, MHG ;
O'Keefe, BR ;
Sowder, RC ;
McKee, TC ;
Currens, MJ ;
Pannell, LK ;
Boyd, MR .
JOURNAL OF NATURAL PRODUCTS, 2000, 63 (08) :1170-1174
[9]   Musa acuminata (Del Monte banana) lectin is a fructose-binding lectin with cytokine-inducing activity [J].
Cheung, Allen H. K. ;
Wong, Jack H. ;
Ng, T. B. .
PHYTOMEDICINE, 2009, 16 (6-7) :594-600
[10]   Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens [J].
Feng, K. ;
Liu, Q. H. ;
Ng, T. B. ;
Liu, H. Z. ;
Li, J. Q. ;
Chen, G. ;
Sheng, H. Y. ;
Xie, Z. L. ;
Wang, H. X. .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 345 (04) :1573-1578
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