Homogeneity of Powassan virus populations in naturally infected Ixodes scapularis

被引：20

作者：

Brackney, Doug E. ^{[1
]}

Brown, Ivy K. ^{[1
]}

Nofchissey, Robert A. ^{[1
]}

Fitzpatrick, Kelly A. ^{[1
]}

Ebel, Gregory D. ^{[1
]}

机构：

[1] Univ New Mexico, Sch Med, Dept Pathol, Albuquerque, NM 87131 USA

来源：

VIROLOGY | 2010年 / 402卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Powassan virus; TBE; Quasispecies; Ixodes scapularis; Genetic diversity; WEST-NILE-VIRUS; DEER TICK VIRUS; STRONG PURIFYING SELECTION; QUASI-SPECIES STRUCTURE; DENGUE TYPE-3 VIRUS; UNITED-STATES; PHYLOGENETIC ANALYSIS; GENETIC-VARIATION; RNA VIRUSES; NEW-ENGLAND;

D O I：

10.1016/j.virol.2010.03.035

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Powassan virus (POWV, Flaviviridae: Flavivirus) is the sole North American member of the tick-borne encephalitis complex and consists of two distinct lineages that are maintained in ecologically discrete enzootic transmission cycles. The underlying genetic mechanisms that lead to niche partitioning in arboviruses are poorly understood. Therefore, intra- and interhost genetic diversity was analyzed to determine if POWV exists as a quasispecies in nature and quantify selective pressures within and between hosts. In contrast to previous reports for West Nile virus (WNV), significant intrahost genetic diversity was not observed. However, pN (0.238) and d(N)/d(S) ratios (0.092) for interhost diversity were similar to those of WNV. Combined, these data suggest that purifying selection and/or population bottlenecks constrain quasispecies diversity within ticks. These same selective and stochastic mechanisms appear to drive minor sequence changes between ticks. Moreover, Powassan virus populations seem not to be structured as quasispecies in naturally infected adult deer ticks. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：366 / 371

页数：6