Among coagulase-positive staphylococci of animal origin, the members of the Staphylococcus intermedius-group (SIG: S.intermedius, Staphylococcus pseudintermedius and Staphylococcus delphini) are important opportunistic pathogens in different animal hosts and occasionally in humans. However, the unambiguous species diagnosis of SIG is often challenging. Therefore, matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS) -based SIG-identification with Bruker Microflex LT in combination with Biotyper 3.0 software (Bruker Daltonics, Bremen, Germany) was evaluated using (i) the original database content and (ii) the database after extension with distinct hierarchical clustered reference spectra for 60 SIG. A convenience sample comprising 200 isolates was used to compare both database performances. As a result, 17 isolates initially diagnosed as S.intermedius with the current content of the Bruker database were identified as S.pseudintermedius by applying the in-house reference spectra extended version. Furthermore, a significant improvement (average rise of log score value: 0.24) of the SIG identification score values was achieved, emphasizing that further sequence-based refinement of the Bruker database content allows improvement of MALDI-TOF MS-based identification.
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Alatoom, Adnan A.
;
Cunningham, Scott A.
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Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Cunningham, Scott A.
;
Ihde, Sherry M.
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Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Ihde, Sherry M.
;
Mandrekar, Jayawant
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Mayo Clin, Div Biomed Stat & Informat, Dept Hlth Sci & Res, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Mandrekar, Jayawant
;
Patel, Robin
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Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Mayo Clin, Div Infect Dis, Dept Med, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
机构:
Univ London Royal Vet Coll, Dept Vet Clin Sci, Hatfield AL9 7TA, Herts, EnglandUniv London Royal Vet Coll, Dept Vet Clin Sci, Hatfield AL9 7TA, Herts, England
Bond, R.
;
Loeffler, A.
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Univ London Royal Vet Coll, Dept Vet Clin Sci, Hatfield AL9 7TA, Herts, EnglandUniv London Royal Vet Coll, Dept Vet Clin Sci, Hatfield AL9 7TA, Herts, England
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Alatoom, Adnan A.
;
Cunningham, Scott A.
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h-index: 0
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Cunningham, Scott A.
;
Ihde, Sherry M.
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h-index: 0
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Ihde, Sherry M.
;
Mandrekar, Jayawant
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h-index: 0
机构:
Mayo Clin, Div Biomed Stat & Informat, Dept Hlth Sci & Res, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Mandrekar, Jayawant
;
Patel, Robin
论文数: 0引用数: 0
h-index: 0
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Mayo Clin, Div Infect Dis, Dept Med, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
机构:
Univ London Royal Vet Coll, Dept Vet Clin Sci, Hatfield AL9 7TA, Herts, EnglandUniv London Royal Vet Coll, Dept Vet Clin Sci, Hatfield AL9 7TA, Herts, England
Bond, R.
;
Loeffler, A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Royal Vet Coll, Dept Vet Clin Sci, Hatfield AL9 7TA, Herts, EnglandUniv London Royal Vet Coll, Dept Vet Clin Sci, Hatfield AL9 7TA, Herts, England