Species differentiation within the Staphylococcus intermedius group using a refined MALDI-TOF MS database

被引:48
作者
Murugaiyan, J. [1 ]
Walther, B. [2 ]
Stamm, I. [3 ]
Abou-Elnaga, Y. [2 ]
Brueggemann-Schwarze, S. [2 ]
Vincze, S. [2 ]
Wieler, L. H. [2 ]
Luebke-Becker, A. [2 ]
Semmler, T. [2 ]
Roesler, U. [1 ]
机构
[1] Free Univ Berlin, Inst Anim Hyg & Environm Hlth, Ctr Infect Med, D-14163 Berlin, Germany
[2] Free Univ Berlin, Inst Microbiol & Epizoot, Ctr Infect Med, D-14163 Berlin, Germany
[3] Vet Med Labor GmbH, Div IDEXX Labs, Ludwigsburg, Germany
关键词
Biotyper; coagulase positive staphylococci; matrix-assisted laser desorption ionizationtime of flight mass spectrometry; microflex; Staphylococcus intermedius-group; Staphylococcus pseudintermedius; FLIGHT MASS-SPECTROMETRY; LASER-DESORPTION IONIZATION; MICROBIOLOGY LABORATORIES; MULTIDRUG-RESISTANT; COMPANION ANIMALS; CLINICAL-SAMPLES; PSEUDINTERMEDIUS; IDENTIFICATION; TIME; EMERGENCE;
D O I
10.1111/1469-0691.12662
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Among coagulase-positive staphylococci of animal origin, the members of the Staphylococcus intermedius-group (SIG: S.intermedius, Staphylococcus pseudintermedius and Staphylococcus delphini) are important opportunistic pathogens in different animal hosts and occasionally in humans. However, the unambiguous species diagnosis of SIG is often challenging. Therefore, matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS) -based SIG-identification with Bruker Microflex LT in combination with Biotyper 3.0 software (Bruker Daltonics, Bremen, Germany) was evaluated using (i) the original database content and (ii) the database after extension with distinct hierarchical clustered reference spectra for 60 SIG. A convenience sample comprising 200 isolates was used to compare both database performances. As a result, 17 isolates initially diagnosed as S.intermedius with the current content of the Bruker database were identified as S.pseudintermedius by applying the in-house reference spectra extended version. Furthermore, a significant improvement (average rise of log score value: 0.24) of the SIG identification score values was achieved, emphasizing that further sequence-based refinement of the Bruker database content allows improvement of MALDI-TOF MS-based identification.
引用
收藏
页码:1007 / 1014
页数:8
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