Polymorphic microsatellite DNA markers from the oriental fruit fly Bactrocera dorsalis (Hendel)

被引:21
作者
Dai, SM
Lin, CC
Chang, C [1 ]
机构
[1] Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 402, Taiwan
[2] Natl Chung Hsing Univ, Grad Inst Entomol, Taichung 402, Taiwan
[3] Natl Chung Hsing Univ, Ctr Biotechnol, Taichung 402, Taiwan
来源
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 04期
关键词
Bactrocera dorsalis (Hendel); heterozygosity; microsatellite; polymerase chain reaction (PCR); primers;
D O I
10.1111/j.1471-8286.2004.00732.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Six polymorphic microsatellite loci are isolated from the Oriental fruit fly Bactrocera dorsalis (Hendel), an agricultural pest in Asia, including Taiwan. To assess their potential utility as high-resolution genetic markers, polymerase chain reaction (PCR) primers, amplification conditions, and an automated fluorescence detection protocol were developed. In analyses of 71 individual flies from six different areas of Taiwan, allele numbers ranged from five to 25 were detected for each locus. The observed heterozygosity ranged between 0.268 and 0.737 among these loci. No linkage disequilibrium was found. These microsatellite markers have potential utility to population structure and gene flow studies of B. dorsalis Mendel).
引用
收藏
页码:629 / 631
页数:3
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