Molecular diagnosis of Anaplasmataceae organisms in dogs with clinical and microscopical signs of ehrlichiosis

被引：34

作者：

Dagnone, Ana Silvia ^{[1
]}

de Souza, Alda Izabel ^{[1
]}

Andre, Marcos Rogerio ^{[1
]}

Machado, Rosangela Zacarias ^{[1
]}

机构：

[1] Univ Estadual Paulista, Lab Imunoparasitol, Dept Patol Vet, Fac Ciencias Agr & Vet Julio Mesquita Filho, BR-14884900 Jaboticabal, SP, Brazil

来源：

REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA | 2009年 / 18卷 / 04期

关键词：

Dogs; Ehrlichia canis; Anaplasma platys; PCR; Brazil; CANINE MONOCYTIC EHRLICHIOSIS; BORRELIA-BURGDORFERI; DIROFILARIA-IMMITIS; INFECTION; PREVALENCE; CHAFFEENSIS; TICKS; THROMBOCYTOPENIA; NEORICKETTSIA; BABESIOSIS;

D O I：

10.4322/rbpv.01804004

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Ehrlichioses are important emerging zoonotic tick-borne diseases that can affect both animals and humans. Clinical manifestations of ehrlichiosis caused by different members of Anaplasmataceae in dogs are similar to each other and to other diseases showing systemic manifestation. The observation of inclusions in white blood cells and in platelets cannot be used to confirm the Anaplasmataceae etiologic agent of the disease. In this work we assessed the presence of Anaplasmataceae agents in 51 dogs from two different cities (Jaboticabal and Campo Grande) showing clinical and microscopical diagnosis of ehrlichiosis, by using molecular techniques. Anaplasmataceae DNA were amplified in 46/51 (90.2%) of the blood samples; 22 (40%) samples from Jaboticabal and 10 (18.2%) from Campo Grande were positive for E. canis nPCR. Anaplasma platys DNA was amplified in 2 samples from Jaboticabal and in 11 from Campo Grande. Phylogenetic analysis of E. cants and A. platys DNA confirmed the infection agent and showed that PCR is the most reliable method to diagnose ehrlichial infection.

引用

页码：20 / 25

页数：6

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