Efficient production by Escherichia coli of recombinant protein using salting-out effect protecting against proteolytic degradation

被引：7

作者：

Kobayashi, F ^{[1
]}

Nakamura, Y ^{[1
]}

机构：

[1] Kanazawa Univ, Fac Engn, Dept Chem & Chem Engn, Kanazawa, Ishikawa 9208667, Japan

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 10期

关键词：

glucoamylase; proteolytic degradation; recombinant Escherichia coli; recombinant protein; salting-out;

D O I：

10.1023/A:1023524520023

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To produce glucoamylase efficiently as a recombinant protein, E. coli was grown with 20 g (NH4)(2)SO4 l(-1) which removed proteolytic activity but did not effect cell growth. Growth in M9 medium with 20 g (NH4)(2)SO4 l(-1) produced 11 U glucoamylase ml(-1) compared to 7 U ml(-1) without addition. Furthermore, the glucoamylase activity was maintained at about 9 U ml(-1).

引用

页码：779 / 782

页数：4

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