Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry

被引：31

作者：

Cho, Soo-Yeon ^{[1
]}

Gong, Xun ^{[1
]}

Koman, Volodymyr B. ^{[1
]}

Kuehne, Matthias ^{[1
]}

Moon, Sun Jin ^{[1
]}

Son, Manki ^{[1
]}

Lew, Tedrick Thomas Salim ^{[1
,2
]}

Gordiichuk, Pavlo ^{[1
]}

Jin, Xiaojia ^{[1
]}

Sikes, Hadley D. ^{[1
]}

Strano, Michael S. ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] ASTAR, Inst Mat Res & Engn IMRE, Singapore, Singapore

来源：

NATURE COMMUNICATIONS | 2021年 / 12卷 / 01期

关键词：

DIFFRACTION TOMOGRAPHY; MOLECULAR RECOGNITION; HYDROGEN-PEROXIDE; U937; CELLS; SINGLE; HETEROGENEITY; MICROSPHERE; SPECIFICITY; MECHANISMS; MONOCYTES;

D O I：

10.1038/s41467-021-23416-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. Herein, we design and fabricate nanosensor array in microfluidics that addresses this limitation, creating a Nanosensor Chemical Cytometry (NCC). nIR fluorescent carbon nanotube array is integrated along microfluidic channel through which flowing cells is guided. We can utilize the flowing cell itself as highly informative Gaussian lenses projecting nIR profiles and extract rich information. This unique biophotonic waveguide allows for quantified cross-correlation of biomolecular information with various physical properties and creates label-free chemical cytometer for cellular heterogeneity measurement. As an example, the NCC can profile the immune heterogeneities of human monocyte populations at attomolar sensitivity in completely non-destructive and real-time manner with rate of similar to 600 cells/hr, highest range demonstrated to date for state-of-the-art chemical cytometry.

引用

页数：13

共 81 条

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