The potential of Raman spectroscopy for the identification of biofilm formation by Staphylococcus epidermidis

被引：69
作者：
Samek, O. [1 ]
Al-Marashi, J. F. M. [2 ]
Telle, H. H. [2 ]
机构：
[1] Acad Sci Czech Republ, Inst Sci Instruments ASCR, VVI, CS-61264 Brno, Czech Republic
[2] Swansea Univ, Dept Phys, Swansea SA2 8PP, W Glam, Wales
来源：
LASER PHYSICS LETTERS | 2010年 / 7卷 / 05期
关键词：
Raman spectroscopy; Staphylococcus epidermidis; biofilm; biofilm-negative; biofilm-positive; principle component analysis; POLYSACCHARIDE INTERCELLULAR ADHESIN; RELEVANT MICROORGANISMS; BACTERIAL; ICA; MECHANISMS; SPECTRA; CELLS;
D O I：
10.1002/lapl.200910154
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We report on an investigation into a common problem in microbiology laboratories, which is associated with the difficulty of distinguishing/recognising different strains of the genus Staphylococcus. We demonstrate the potential, of, Raman spectroscopy as a rapid techniques allowing for the identification of different isolates for the detection of biofilin-positive and biofilm-negative Staphylococcus epidermidis strains, For this, the recorded Spectra were interpreted using the approach of principal component analysis (PCA). [GRAPHICS] Typical Raman spectra from individual bacterial colonies for three S. epidermidis strains (C) 2010 by Astro Ltd Published exclusively by WILEY-VCH Verlag GmbH & Co KGaA
引用
收藏
页码：378 / 383
页数：6
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