Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon

被引:89
作者
Braun, D [1 ]
Fromherz, P [1 ]
机构
[1] MAX PLANCK INST BIOCHEM, DEPT MEMBRANE & NEUROPHYS, D-82152 MARTINSRIED, GERMANY
来源
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING | 1997年 / 65卷 / 4-5期
关键词
D O I
10.1007/s003390050589
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Standing modes of light in front of the reflecting surface of silicon modulate the excitation and emission of fluorescent dyes. This effect was used to determine the distance of a biomembrane from an oxidized silicon chip. The membrane of a red blood cell (ghost) was stained with a cyanine dye and attached with poly-lysine to a surface structured with microscopic steps of silicon dioxide on silicon. The system was illuminated in a microscope. The fluorescence intensity of the membrane depended on the height of the steps. The data were fitted by an optical theory which accounts both for the interference of the exciting light and for the interference of the emitted light at a finite aperture. The distance between the membrane and the silicon dioxide was determined to be 12 nm.
引用
收藏
页码:341 / 348
页数:8
相关论文
共 40 条
[1]   CELL-SUBSTRATE CONTACTS ILLUMINATED BY TOTAL INTERNAL-REFLECTION FLUORESCENCE [J].
AXELROD, D .
JOURNAL OF CELL BIOLOGY, 1981, 89 (01) :141-145
[2]   CARBOCYANINE DYE ORIENTATION IN RED-CELL MEMBRANE STUDIED BY MICROSCOPIC FLUORESCENCE POLARIZATION [J].
AXELROD, D .
BIOPHYSICAL JOURNAL, 1979, 26 (03) :557-573
[3]   TOTAL INTERNAL-REFLECTION FLUORESCENT MICROSCOPY [J].
AXELROD, D ;
THOMPSON, NL ;
BURGHARDT, TP .
JOURNAL OF MICROSCOPY-OXFORD, 1983, 129 (JAN) :19-28
[4]   DIGITAL IMAGING FLUORESCENCE MICROSCOPY - SPATIAL HETEROGENEITY OF PHOTOBLEACHING RATE CONSTANTS IN INDIVIDUAL CELLS [J].
BENSON, DM ;
BRYAN, J ;
PLANT, AL ;
GOTTO, AM ;
SMITH, LC .
JOURNAL OF CELL BIOLOGY, 1985, 100 (04) :1309-1323
[5]  
Born M., 1986, PRINCIPLES OPTICS
[6]   QUANTITATIVE-ANALYSIS OF VARIABLE-ANGLE TOTAL INTERNAL-REFLECTION FLUORESCENCE MICROSCOPY (VA-TIRFM) OF CELL SUBSTRATE CONTACTS [J].
BURMEISTER, JS ;
TRUSKEY, GA ;
REICHERT, WM .
JOURNAL OF MICROSCOPY-OXFORD, 1994, 173 :39-51
[8]  
DONATH E, 1983, J CELL SCI, V63, P113
[9]   A NEURON-SILICON JUNCTION - A RETZIUS CELL OF THE LEECH ON AN INSULATED-GATE FIELD-EFFECT TRANSISTOR [J].
FROMHERZ, P ;
OFFENHAUSSER, A ;
VETTER, T ;
WEIS, J .
SCIENCE, 1991, 252 (5010) :1290-1293
[10]   SILICON-NEURON JUNCTION - CAPACITIVE STIMULATION OF AN INDIVIDUAL NEURON ON A SILICON CHIP [J].
FROMHERZ, P ;
STETT, A .
PHYSICAL REVIEW LETTERS, 1995, 75 (08) :1670-1673