Determination of ginsenosides in Panax ginseng roots by liquid chromatography with evaporative light-scattering detection

被引:1
作者
Fuzzati, N [1 ]
Gabetta, B [1 ]
Jayakar, K [1 ]
Pace, R [1 ]
Ramaschi, G [1 ]
Villa, F [1 ]
机构
[1] Indena SPA, Res & Dev Labs, I-20090 Milan, Italy
关键词
D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A liquid chromatographic method was developed and validated for the determination of ginsenosides in Panax ginseng roots by using evaporative light-scattering detection. Eighteen ginsenosides were separated on a reversed-phase C-18 column with water-ammonium acetate-acetonitrile as the mobile phase. The method is suitable for the routine determination of ginsenosides in P, ginseng roots and extracts. The validation of the method was comprehensive for efficiency and recovery optimization of the P. ginseng roots extraction, specificity by liquid chromatography/mass spectrometry, linearity, stability, reproducibility, repeatability, intermediate precision, and robustness.
引用
收藏
页码:820 / 829
页数:10
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