Mass Spectrometry Reveals Molecular Structure of Polyhydroxyalkanoates Attained by Bioconversion of Oxidized Polypropylene Waste Fragments

被引：33

作者：

Johnston, Brian ^{[1
]}

Radecka, Iza ^{[1
]}

Chiellini, Emo ^{[2
]}

Barsi, David ^{[2
]}

Ilieva, Vassilka Ivanova ^{[2
]}

Sikorska, Wanda ^{[3
]}

Musiol, Marta ^{[3
]}

Zieba, Magdalena ^{[3
]}

Chaber, Pawel ^{[3
]}

Marek, Adam A. ^{[4
]}

Mendrek, Barbara ^{[1
,3
]}

Ekere, Anabel Itohowo ^{[1
]}

Adamus, Grazyna ^{[3
]}

Kowalczuk, Marek ^{[1
,3
]}

机构：

[1] Univ Wolverhampton, Fac Sci & Engn, Wolverhampton Sch Biol Chem & Forens Sci, Wolverhampton WV1 1LY, W Midlands, England

[2] LMPE, Via Nuova 44-A, I-55018 Capannori, LU, Italy

[3] Polish Acad Sci, Ctr Polymer & Carbon Mat, PL-41800 Zabrze, Poland

[4] Silesian Tech Univ, Dept Chem Organ Technol & Petrochem, PL-44100 Gliwice, Poland

来源：

POLYMERS | 2019年 / 11卷 / 10期

基金：

欧盟地平线“2020”;

关键词：

electrospray ionization dual mass spectrometry (ESI-MS/MS); polyhydroxyalkanoate (PHA); polypropylene (PP); prodegraded; Cupriavidus necator; coral reef; bioplastics; recycling; SEQUENCE DISTRIBUTION; LEVEL STRUCTURE; DEGRADATION; BIOPOLYESTERS; COPOLYESTERS; POLYETHYLENE; POLYESTERS;

D O I：

10.3390/polym11101580

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

This study investigated the molecular structure of the polyhydroxyalkanoate (PHA) produced via a microbiological shake flask experiment utilizing oxidized polypropylene (PP) waste as an additional carbon source. The bacterial strain Cupriavidus necator H16 was selected as it is non-pathogenic, genetically stable, robust, and one of the best known producers of PHA. Making use of PHA oligomers, formed by controlled moderate-temperature degradation induced by carboxylate moieties, by examination of both the parent and fragmentation ions, the ESI-MS/MS analysis revealed the 3-hydroxybutyrate and randomly distributed 3-hydroxyvalerate as well as 3-hydroxyhexanoate repeat units. Thus, the bioconversion of PP solid waste to a value-added product such as PHA tert-polymer was demonstrated.

引用

页数：20

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