Aquaporin-3 is down-regulated in jejunum villi epithelial cells during enterotoxigenic Escherichia coli-induced diarrhea in mice

被引：18

作者：

Zhang, Di ^{[1
]}

Zhang, Kaiqi ^{[1
]}

Su, Weiheng ^{[2
]}

Zhao, Yuan ^{[1
]}

Ma, Xin ^{[1
]}

Qian, Gong ^{[1
]}

Qu, Guijuan ^{[1
]}

Pei, Zhihua ^{[1
]}

Liu, Shuming ^{[1
]}

Ma, Hongxia ^{[1
]}

机构：

[1] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China

[2] Jilin Univ, Sch Life Sci, Natl Engn Lab AIDS Vaccine, Changchun 130012, Peoples R China

来源：

MICROBIAL PATHOGENESIS | 2017年 / 107卷

基金：

中国国家自然科学基金;

关键词：

Aquaporin-3; Enterotoxigenic Escherichia coli; Diarrhea; Small intestine; WATER CHANNEL; EXPRESSION; MECHANISMS; TRANSPORT; INTESTINE;

D O I：

10.1016/j.micpath.2017.04.031

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Enterotoxigenic Escherichia coli (ETEC)-induced diarrhea is a complex pathological process, involving ion channel regulation and water efflux. While the mechanism underlying water efflux in ETEC-induced diarrhea is still largely unknown, aquaporins (AQPs) play important roles in transcellular water movement, but their expression profile has not been demonstrated in the murine small intestine. We identified AQP3 expression in the jejunum, but not the duodenum or ileum, using reverse transcription PCR and western blotting. Immunohistochemistry showed that AQP3 localized to the jejunum villi epithelial cells. Using an ETEC-induced murine diarrhea model, we demonstrated that both AQP3 mRNA expression and protein concentration in the jejunum were gradually but significantly decreased over 7 d compared with controls. These results suggested impaired water influx also plays an important role in ETEC-induced diarrhea. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：430 / 435

页数：6

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