Changes in Nutritive Value and Digestion Kinetics of Canola Seed Due to Microwave Irradiation

被引:17
作者
Ebrahimi, S. R. [1 ]
Nikkhah, A. [1 ]
Sadeghi, A. A. [1 ]
机构
[1] Islamic Azad Univ, Dept Anim Sci, Fac Agr, Shahr e Qods Branch, Tehran, Iran
来源
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2010年 / 23卷 / 03期
关键词
Canola Seed; Microwave Irradiation; Protein Degradation; SDS-PAGE; Glucosinolate; RUMINAL PROTEIN-DEGRADATION; INTESTINAL DIGESTIBILITY; CHEMICAL-COMPOSITION; GAMMA-IRRADIATION; RAPESEED MEAL; ANTINUTRITIONAL FACTORS; AMINO-ACIDS; DAIRY-COWS; DEGRADABILITY; PERFORMANCE;
D O I
10.5713/ajas.2010.80574
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
This study aimed to evaluate effects of 800 W microwave irradiation for 2, 4 and 6 min on chemical composition. anti-nutritional factors, ruminal dry matter (DM) and crude protein (CP) degradability, and in vitro CP digestibility of canola seed (CS). Nylon bags of untreated or irradiated CS were suspended in the rumen of three bulls from 0 to 48 h. Protein subfractions of untreated and microwave irradiated CS before and after incubation in the rumen were monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Microwave irradiation had no effect on chemical composition of CS (p>0.05). There was a linear decrease (p<0.001) in the phytic acid and glucosinolate contents of CS as irradiation time increased. Microwave irradiation for 2, 4 and 6 min decreased the phytic acid content of CS by 8.2, 27.6 and 48.6%, respectively. The total glucosinolate contents of CS microwave irradiated for 2, 4 and 6 min decreased by 41.5, 54.7 and 59.0% respectively, compared to untreated samples. The washout fractions of DM and CP and degradation rate of the b fraction of CP decreased linearly (p<0.001) as irradiation time increased. Microwave irradiation for 2, 4 and 6 min decreased effective degradability (ED) of CP at a ruminal outflow rate of 0.05 h(-1) by 4.7, 12.3 and 2 1.0%, respectively Microwave irradiation increased linearly (p<0.001) in vitro CP digestibility of ruminally undegraded CS collected after 16 h incubation. Electrophoresis results showed that napin subunits of untreated CS disappeared completely within the zero incubation period. whereas cruciferin subunits were degraded in the middle of the incubation period (16 h incubation period). In 4 and 6 min microwave irradiated CS, napin subunits were degraded after 4 and 16 h incubation periods, respectively, and cruciferin subunits were not degraded untile 24 h of incubation. In conclusion, it seems that microwave irradiation not only protected CP of CS from ruminal degradation, but also increased in vitro digestibility of CP. Moreover, microwave irradiation was effective in reducing glucosinolate and phytic acid contents of CS.
引用
收藏
页码:347 / 354
页数:8
相关论文
共 52 条
[1]   Nutritional composition of chickpea microwave cooking and other (Cicer arietinum L.) as affected by traditional cooking methods [J].
Alajaji, Saleh A. ;
El-Adawy, Tarek A. .
JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2006, 19 (08) :806-812
[2]  
[Anonymous], 1996, NUTR REQ BEEF CATTL
[3]  
[Anonymous], 1995, OFF METH AN, V16th
[4]   Effects of pressure toasting on in situ degradability and intestinal protein and protein-free organic matter digestibility of rapeseed [J].
Azarfar, Arash ;
Ferreira, Claudio S. ;
Goelema, Jacob O. ;
Van der Poel, Antonius F. B. .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2008, 88 (08) :1380-1384
[5]   Bioeffects of microwave - a brief review [J].
Banik, S ;
Bandyopadhyay, S ;
Ganguly, S .
BIORESOURCE TECHNOLOGY, 2003, 87 (02) :155-159
[6]   IDENTIFICATION AND PROPERTIES OF PHYTATE IN CEREAL-GRAINS AND OILSEED PRODUCTS [J].
BOLAND, ARD ;
GARNER, GB ;
ODELL, BL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1975, 23 (06) :1186-1189
[7]  
BRODERICK GA, 1991, PHYSL ASPECTS DIGEST, P542
[8]  
CALSAMIGLIA S, 1995, J ANIM SCI, V73, P1459
[9]   Effects of feeding extruded soybean, ground canola seed and whole cottonseed on ruminal fermentation, performance and milk fatty acid profile in early lactation-dairy cows [J].
Chen, P. ;
Ji, P. ;
Li, S. L. .
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 2008, 21 (02) :204-213
[10]   Separation of phytic acid and other related inositol phosphates by high-performance ion chromatography and its applications [J].
Chen, QC ;
Li, BW .
JOURNAL OF CHROMATOGRAPHY A, 2003, 1018 (01) :41-52