Dominance of candidate Saccharibacteria in a membrane bioreactor treating medium age landfill leachate: Effects of organic load on microbial communities, hydrolytic potential and extracellular polymeric substances

被引：74

作者：

Remmas, Nikolaos ^{[1
]}

Melidis, Paraschos ^{[1
]}

Zerva, Ioanna ^{[1
]}

Kristoffersen, Jon Bent ^{[2
]}

Nikolaki, Sofia ^{[3
]}

Tsiamis, George ^{[3
]}

Ntougias, Spyridon ^{[1
]}

机构：

[1] Democritus Univ Thrace, Dept Environm Engn, Lab Wastewater Management & Treatment Technol, Vas Sofias 12, GR-67100 Xanthi, Greece

[2] Hellenic Ctr Marine Res, Inst Marine Biol Biotechnol & Aquaculture, Iraklion 71500, Greece

[3] Univ Patras, Dept Environm & Nat Resources Management, 2 Seferi St, Agrinion 30100, Greece

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 238卷

关键词：

Candidate phylum Saccharibacteria (candidate division TM7); Cryptomycota (Rozellomycota); Candidate phylum Dojkabacteria (candidate division WS6); beta-glucosidase; Membrane fouling; WASTE-WATER TREATMENT; SEQUENCING BATCH REACTOR; BACTERIAL COMMUNITY; SALINITY; DIVERSITY; BIODEGRADATION; PERFORMANCE; RETENTION; BIOMASS; TM7;

D O I：

10.1016/j.biortech.2017.04.019

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A membrane bioreactor (MBR), accomplishing high nitrogen removal efficiencies, was evaluated under various landfill leachate concentrations (50, 75 and 100% v/v). Proteinous and carbohydrate extracellular polymeric substances (EPS) and soluble microbial product (SMP) were strongly correlated (p < 0.01) with organic load, salinity and NH4+-N. Exceptionally high beta-glucosidase activities (6700-10,100 U g (1)) were determined during MBR operation with 50% v/v leachate, as a result of the low organic carbon availability that extendedly induced beta-glucosidases to breakdown the least biodegradable organic fraction. Illumina sequencing revealed that candidate Saccharibacteria were dominant, independently of the leachate concentration applied, whereas other microbiota (21.2% of total reads) disappeared when undiluted leachate was used. Fungal taxa shifted from a Saccharomyces- to a newly-described Cryptomycota-based community with increasing leachate concentration. Indeed, this is the first report on the dominance of candidate Saccharibacteria and on the examination of their metabolic behavior in a bioreactor treating real wastewater. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：48 / 56

页数：9

共 35 条

[1] Membrane fouling properties in a submerged membrane bioreactor for saline wastewater treatment at high ammonium content [J].