Ultra-high-performance liquid chromatography for the characterization of therapeutic proteins

被引：48
作者：
Fekete, Szabolcs [1 ]
Guillarme, Davy [1 ]
机构：
[1] Univ Geneva, Univ Lausanne, Sch Pharmaceut Sci, CH-1211 Geneva 4, Switzerland
来源：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2014年 / 63卷
关键词：
Biopharmaceutical; Characterization; Hydrophilic interaction chromatography (HILIC); Ion-exchange chromatography (IEX); Mass spectrometry (MS); Monoclonal antibody (mAb); Reversed-phase liquid chromatography (RPLC); Size-exclusion chromatography (SEC); Therapeutic protein; Ultra-high-performance liquid chromatography (UHPLC); SUPERFICIALLY POROUS PARTICLES; MONOCLONAL-ANTIBODIES; NEW-GENERATION; PEPTIDE SEPARATIONS; STATIONARY PHASES; N-GLYCANS; PRESSURE; COLUMN; IDENTIFICATION; SPECTROMETRY;
D O I：
10.1016/j.trac.2014.05.012
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The pharmaceutical market has markedly changed over the past few years, and there are today an increasing number of therapeutic drugs produced from biological sources. These biopharmaceuticals include recombinant peptides, proteins, and monoclonal antibodies (mAbs). Their detailed characterization could be difficult and time consuming, so it requires powerful chromatographic and spectroscopic methods. In this context, the use of columns packed with sub-2-mu m particles at very high pressure, also known as ultra-high-performance (or pressure) liquid chromatography (UHPLC) has been reported as successful. Various modes of chromatography are compatible with UHPLC columns and conditions, including reversed-phase liquid chromatography (RPLC), size-exclusion chromatography (SEC), ion-exchange chromatography (IEX) and hydrophilic interaction chromatography (HILIC). (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：76 / 84
页数：9
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