Glucose-6-Phosphate Regulates Hepatic Bile Acid Synthesis in Mice

被引:25
作者
Hoogerland, Joanne A. [1 ]
Lei, Yu [1 ]
Wolters, Justina C. [1 ]
de Boer, Jan Freark [1 ,2 ]
Bos, Trijnie [1 ]
Bleeker, Aycha [1 ]
Mulder, Niels L. [1 ]
van Dijk, Theo H. [2 ]
Kuivenhoven, Jan A. [1 ]
Rajas, Fabienne [3 ]
Mithieux, Gilles [3 ]
Haeusler, Rebecca A. [4 ]
Verkade, Henkjan J. [1 ]
Bloks, Vincent W. [1 ]
Kuipers, Folkert [1 ,2 ]
Oosterveer, Maaike H. [1 ]
机构
[1] Univ Groningen, Univ Med Ctr Groningen, Dept Pediat, Groningen, Netherlands
[2] Univ Groningen, Univ Med Ctr Groningen, Lab Med, Groningen, Netherlands
[3] Univ Claude Bernard Lyon, INSERM, U1213, Villeurbanne, France
[4] Columbia Univ Coll Phys & Surg, Dept Pathol & Cell Biol, 630 W 168th St, New York, NY 10032 USA
关键词
FARNESOID-X RECEPTOR; INSULIN-RESISTANCE; GENE-EXPRESSION; DEFICIENT MICE; GLUCOSE; CHREBP; LIVER; METABOLISM; TRANSCRIPTION; PURIFICATION;
D O I
10.1002/hep.30778
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
It is well established that, besides facilitating lipid absorption, bile acids act as signaling molecules that modulate glucose and lipid metabolism. Bile acid metabolism, in turn, is controlled by several nutrient-sensitive transcription factors. Altered intrahepatic glucose signaling in type 2 diabetes associates with perturbed bile acid synthesis. We aimed to characterize the regulatory role of the primary intracellular metabolite of glucose, glucose-6-phosphate (G6P), on bile acid metabolism. Hepatic gene expression patterns and bile acid composition were analyzed in mice that accumulate G6P in the liver, that is, liver-specific glucose-6-phosphatase knockout (L-G6pc(-/-)) mice, and mice treated with a pharmacological inhibitor of the G6P transporter. Hepatic G6P accumulation induces sterol 12 alpha-hydroxylase (Cyp8b1) expression, which is mediated by the major glucose-sensitive transcription factor, carbohydrate response element-binding protein (ChREBP). Activation of the G6P-ChREBP-CYP8B1 axis increases the relative abundance of cholic-acid-derived bile acids and induces physiologically relevant shifts in bile composition. The G6P-ChREBP-dependent change in bile acid hydrophobicity associates with elevated plasma campesterol/cholesterol ratio and reduced fecal neutral sterol loss, compatible with enhanced intestinal cholesterol absorption. Conclusion: We report that G6P, the primary intracellular metabolite of glucose, controls hepatic bile acid synthesis. Our work identifies hepatic G6P-ChREBP-CYP8B1 signaling as a regulatory axis in control of bile acid and cholesterol metabolism.
引用
收藏
页码:2171 / 2184
页数:14
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