MicroRNA 3113-5p is a novel marker for early cardiac ischemia/reperfusion injury

被引:11
作者
Chen, Yuanyuan [1 ,2 ]
Ye, Xing [1 ,3 ]
Yan, Fengping [1 ,4 ]
机构
[1] Gannan Med Coll, Dept Forens Med, Sch Basic Med Sci, 1 Yixueyuan Rd, Ganzhou 341000, Jiangxi, Peoples R China
[2] Acad Forens Sci, Minist Justice China, Shanghai 200063, Peoples R China
[3] Fudan Univ, Sch Basic Med Sci, Dept Forens Med, Shanghai 200032, Peoples R China
[4] Gannan Med Coll, Key Lab Prevent & Treatment Cardiovasc & Cerebrov, Minist Educ, Ganzhou 341000, Jiangxi, Peoples R China
关键词
Ischemia; reperfusion injury; Heart; Cardiac biomarkers; microRNA; 3113-5p; CIRCULATING MICRORNAS; MYOCARDIAL-INFARCTION; NITRIC-OXIDE; EXPRESSION; REPERFUSION; DECREASES; DIAGNOSIS; ISCHEMIA; RNA;
D O I
10.1186/s13000-019-0894-1
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Background Ischemia/reperfusion (I/R) injury of heart is one of the major causes of acute cardiac injury, which may result in worsening or even loss of heart function. With novel microRNAs being evolutionarily discovered, numbers of them remained functionally unknown. We aimed to discover novel microRNAs with therapeutic or diagnostic potential in the setting of early cardiac I/R injury. Methods Cardiac electrical activity, biochemical detection and histopathology analysis were performed to reveal early changes of cardiac I/R injury. A microRNA array was performed to screen differential microRNAs in the mouse model of cardiac I/R injury. The differentially expressed microRNAs were validated in cardiac tissues and in serum samples. Results The abnormality in electrocardiogram and increases in serum cTnI levels suggested the successful establishment of cardiac I/R injury in mice. A total of 1882 microRNAs were identified, of which 11 were significantly down-regulated and 41 were significantly up-regulated at 3 h post reperfusion. microRNA 223-3p and microRNA 3113-5p were among the mostly altered microRNAs and were validated to be up-regulated within the early hours of I/R injury in heart tissues. In the circulating system, cTnI, a sensitive marker of cardiac injury, or microRNA 223-3p only increased within the first 6 h post I/R injury. However, microRNA 3113-5p stably increased in the serum, keeping an increase of 2.5-fold throughout the 24 h. In the human serum samples, microRNA 3113-5p was detected to be significantly upregulated as soon as 3 h after I/R stimuli and kept significantly higher levels within the 48 h. Conclusion This is the first study that reported the functional roles of microRNA 3113-5p in cardiovascular system. Our data suggested that cardiac microRNA 3113-5p might be a useful target for therapeutic purposes and circulating microRNA 3113-5p might serve as a stable marker for early diagnosis of cardiac I/R injury.
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页数:11
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