Label-free analysis of senescent cells by light sheet microfluidic cytometry with a disposable hydrodynamic focusing unit

被引：0
作者：
Lin, Meiai [1 ]
Zhang, Shuyu [1 ]
Liu, Qiao [2 ]
Shao, Changshun [2 ]
Su, Xuantao [1 ]
机构：
[1] Shandong Univ, Sch Control Sci & Engn, Inst Biomed Engn, Jinan 250061, Shandong, Peoples R China
[2] Shandong Univ, Sch Basic Med Sci, Dept Mol Med & Genet, Jinan 250012, Shandong, Peoples R China
来源：
14TH NATIONAL CONFERENCE ON LASER TECHNOLOGY AND OPTOELECTRONICS (LTO 2019) | 2019年 / 11170卷
基金：
中国国家自然科学基金;
关键词：
Microfluidic cytometry; light sheet; label free; light scattering; tumor cell senescence; single cell analysis; LEUKEMIC-CELLS; CLASSIFICATION;
D O I：
10.1117/12.2534145
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
The detection of senescent cells becomes increasing important for tumor therapy and drug screening. Here a light sheet microfluidic cytometer with a disposable hydrodynamic focusing unit is developed for two dimensional (2D) light scattering measurements of single cells. The mixed polystyrene microspheres of 3.87 and 2.0 mu m in diameter are successfully differentiated by our 2D light scattering microfluidic cytometer. The application of the 2D light scattering microfluidic cytometry for the label-free analysis of senescent cells without any labeling or staining is demonstrated by measurements of H2O2-treated U87 cells. Our light sheet-based 2D light scattering microfluidic cytometer is easy to assemble with a disposable hydrodynamic unit, which may find wild applications in clinics for label-free cell classification.
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页数：6
相关论文
共 17 条
  • [1] Numerical investigation of two-dimensional light scattering patterns of cervical cell nuclei to map dysplastic changes at different epithelial depths
    Arifler, Dizem
    MacAulay, Calum
    Follen, Michele
    Guillaud, Martial
    [J]. BIOMEDICAL OPTICS EXPRESS, 2014, 5 (02): : 485 - 498
  • [2] Microfluidic Impedance-Based Flow Cytometry
    Cheung, Karen C.
    Di Berardino, Marco
    Schade-Kampmann, Grit
    Hebeisen, Monika
    Pierzchalski, Arkadiusz
    Bocsi, Jozsef
    Mittag, Anja
    Tarnok, Attila
    [J]. CYTOMETRY PART A, 2010, 77A (07) : 648 - 666
  • [3] Cellular senescence in aging and age-related disease: from mechanisms to therapy
    Childs, Bennett G.
    Durik, Matej
    Baker, Darren J.
    van Deursen, Jan M.
    [J]. NATURE MEDICINE, 2015, 21 (12) : 1424 - 1435
  • [4] Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo
    Debacq-Chainiaux, Florence
    Erusalimsky, Jorge D.
    Campisi, Judith
    Toussaint, Olivier
    [J]. NATURE PROTOCOLS, 2009, 4 (12) : 1798 - 1806
  • [5] Senescence in Health and Disease
    He, Shenghui
    Sharpless, Norman E.
    [J]. CELL, 2017, 169 (06) : 1000 - 1011
  • [6] Label-free light-sheet microfluidic cytometry for the automatic identification of senescent cells
    Lin, Meiai
    Liu, Qiao
    Liu, Chao
    Qiao, Xu
    Shao, Changshun
    Su, Xuantao
    [J]. BIOMEDICAL OPTICS EXPRESS, 2018, 9 (04): : 1692 - 1703
  • [7] Light-sheet-based 2D light scattering cytometry for label-free characterization of senescent cells
    Lin, Meiai
    Qiao, Xu
    Liu, Qiao
    Shao, Changshun
    Su, Xuantao
    [J]. BIOMEDICAL OPTICS EXPRESS, 2016, 7 (12): : 5170 - 5181
  • [8] Roninson IB, 2003, CANCER RES, V63, P2705
  • [9] Tumor senescence as a determinant of drug response in vivo
    Roninson, IB
    [J]. DRUG RESISTANCE UPDATES, 2002, 5 (05) : 204 - 208
  • [10] The evolution of cytometers
    Shapiro, HM
    [J]. CYTOMETRY PART A, 2004, 58A (01) : 13 - 20
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