Nucleic acid aptamer application in diagnosis and therapy of colorectal cancer based on cell-SELEX technology

被引：68
作者：
Chen, Chan [1 ,2 ]
Zhou, Shan [1 ,2 ]
Cai, Yongqiang [1 ,2 ]
Tang, Faqing [1 ,2 ,3 ,4 ]
机构：
[1] Jinan Univ, Zhuhai Peoples Hosp, Clin Lab, Zhuhai Hosp, Zhuhai 519000, Guangdong, Peoples R China
[2] Jinan Univ, Zhuhai Peoples Hosp, Med Res Ctr, Zhuhai Hosp, Zhuhai 519000, Guangdong, Peoples R China
[3] Cent South Univ, Xiangya Sch Med, Clin Lab, Hunan Canc Hosp, Changsha 410006, Hunan, Peoples R China
[4] Cent South Univ, Xiangya Sch Med, Affiliated Canc Hosp, Changsha 410006, Hunan, Peoples R China
来源：
NPJ PRECISION ONCOLOGY | 2017年 / 1卷
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
MESOPOROUS SILICA NANOPARTICLES; IN-VITRO SELECTION; DRUG-DELIVERY; SYSTEMATIC EVOLUTION; HIGH-AFFINITY; DNA APTAMERS; COMBINATORIAL SELECTION; EXPONENTIAL ENRICHMENT; MOLECULAR RECOGNITION; ADHESION MOLECULE;
D O I：
10.1038/s41698-017-0041-y
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Nucleic acid aptamers are a class of high-affinity nucleic acid ligands. They serve as "chemical antibodies" since their high affinity and specificity. Nucleic acid aptamers are generated from nucleic acid random-sequence using a systematic evolution of ligands by exponential enrichment (SELEX) technology. SELEX is a process of effectively selecting aptamers from different targets. A newly developed cell-based SELEX technique has been widely used in biomarker discovery, early diagnosis and targeted cancer therapy, particular at colorectal cancer (CRC). Combined with nanostructures, nano-aptamer-drug delivery system was constructed for drug delivery. Various nanostructures functionalized with aptamers are highly efficient and has been used in CRC therapeutic applications. In the present, we introduce a cell-SELEX technique, and summarize the potential application of aptamers as biomarkers in CRC diagnosis and therapy. And some characteristics of aptamer-targeted nanocarriers in CRC have been expatiated. The challenges and perspectives for cell-SELEX are also discussed.
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页数：7
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