Two-photon excitation fluorescence microscopy

被引：896

作者：

So, PTC ^{[1
]}

Dong, CY

Masters, BR

Berland, KM

机构：

[1] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

[2] Emory Univ, Dept Phys, Atlanta, GA 30322 USA

[3] Univ Bern, Dept Ophthalmol, Bern, Switzerland

来源：

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING | 2000年 / 2卷

关键词：

multiphoton; fluorescence spectroscopy; single molecule; functional imaging; tissue imaging;

D O I：

10.1146/annurev.bioeng.2.1.399

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.

引用

页码：399 / 429

页数：39

共 167 条

[1] Design of organic molecules with large two-photon absorption cross sections [J].