Determination of xanthohumol in hops (Humulus lupulus L.) by nonaqueous CE

被引：12

作者：

Kac, Javor

Zakrajsek, Jure

Mlinaric, Ales

Kreft, Samo

Filipic, Metka

机构：

[1] Univ Ljubljana, Fac Pharm, SI-1000 Ljubljana, Slovenia

[2] Javni Zdravstveni Zavod Mariborske Iekarne, Maribor, Slovenia

[3] Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia

来源：

ELECTROPHORESIS | 2007年 / 28卷 / 06期

关键词：

hops; Humulus lupulus; NACE; prenylflavonoids; xanthohumol;

D O I：

10.1002/elps.200600494

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Xanthohumol (XN) is a prenylated chalcone with antimutagenic and anticancer activity from hops. A nonaqueous reverse polarity capillary electrophoretic method for the determination of XN in hop extract was developed and validated. The optimal parameters were a 64.5 cm long fused-silica capillary with 50 mu m id at 25 degrees C; 30 kV negative voltage (anode at detector side of the capillary); nonaqueous buffer with 75 mM NaOH and 50 mM boric acid in methanol; hydrodynamical injection with 10 mbar for 40 s; and detection at 440 nm. XN, isoxanthohumol (IX), colupulone, adlupulone, and n-lupulone were well resolved on the electropherogram. The LOD for XN was 0.05 mg/L and RSD for peak area was below 3%. The amount of XN in different samples of hop pellets varied from 0.14 to 0.42%.

引用

页码：965 / 969

页数：5

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