Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.

被引:18
作者
Emsen, Bugrahan [1 ]
Ozdemir, Ozlem [2 ]
Engin, Tubanur [3 ]
Togar, Basak [4 ]
Cavusoglu, Seyda [5 ]
Turkez, Hasan [2 ,6 ]
机构
[1] Karamanoglu Mehmetbey Univ, Kamil Ozdag Fac Sci, Dept Biol, Ibrahim Oktem St, TR-70200 Karaman, Turkey
[2] Erzurum Tech Univ, Fac Sci, Dept Mol Biol & Genet, Airport Rd St, TR-25050 Erzurum, Turkey
[3] Kafkas Univ, Fac Vet Med, Dept Physiol, Pasacayiri Campus, TR-36100 Kars, Turkey
[4] Bayburt Univ, Vocat Sch Hlth Serv, Dept Med Serv & Tech, 21 February St,Dede Korkut Campus, TR-69000 Bayburt, Turkey
[5] Van Yuzuncu Yil Univ, Fac Agr, Dept Hort, TR-65090 Tusba, Van, Turkey
[6] Univ G dAnnunzio, Dept Pharm, Via Vestini 31, I-66100 Chieti, Italy
来源
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2019年 / 91卷 / 03期
关键词
cytotoxicity; genotoxicity; HPLC; total antioxidant capacity; total oxidative stress; USNIC ACID; IN-VITRO; DIFFRACTAIC ACID; ANTICANCER ACTIVITIES; CYTOTOXIC ACTIVITIES; PHENOLIC-COMPOUNDS; LICHEN COMPOUNDS; PROTECTIVE ROLE; ANTIOXIDANT; EXTRACT;
D O I
10.1590/0001-3765201920180994
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2'-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed glial-neuronal non-cancerous cells from Sprague-Dawley rats were also utilized to measure the effects of treatments on non-cancerous cells. Based on median inhibitory concentration values, the data belonged to non-cancerous cells (2486.71 mg/L) showed distinct towering compared to U87MG (80.93 mg/L) cells. The viability of non-cancerous and U87MG cells exposed to extract is decreased in a dose dependent manner. It was also showed that low concentrations of extract notably increased total antioxidant capacity on non-cancerous cells. In addition, various phenolic compounds in extract were detected through high-perfonnance liquid chromatography. The recent results encourage that extract will be able to have therapeutic potential against glioblastoma.
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页数:14
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