Telomerase activity in human leukemic cell lines is inhibited by antisense pentadecadeoxynucleotides targeted against c-myc mRNA

被引:48
作者
Fujimoto, K [1 ]
Takahashi, M [1 ]
机构
[1] Kanazawa Univ, Canc Res Inst, Dept Cellular & Mol Biol, Kanazawa, Ishikawa 920, Japan
关键词
D O I
10.1006/bbrc.1997.7806
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Telomerase is a ribonucleoprotein complex that is thought to add telomeric repeats to the ends of chromosomes during the replicative phase of the cell cycle. We tested the hypothesis that proto-oncogene c-myc plays an essential role in the regulation of telomerase activity in vertebrate cells by exposing three human leukemic cell lines, HL60, U937, and K562, to 15-mer antisense c-myc oligonucleotides. All the treated cells showed a profound decrease in telomerase activity after c-myc antisense oligomer treatment, whereas cells treated with c-myc sense oligomers showed essentially no change in telomerase activity. (C) 1997 Academic Press.
引用
收藏
页码:775 / 781
页数:7
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