Bacillus zhangzhouensis sp nov and Bacillus australimaris sp nov.

Two strains, designated DW5-4(T) and NH7I_1(T), were isolated from aquaculture water of a shrimp farm of Zhangzhou city in China and from surface sediment of the South China Sea, respectively. Cells of the strains were Gram-stain-positive, oxidase-and catalase-positive, rod-shaped and motile. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to the Bacillus pumilus clade. 16S rRNA gene sequence similarity between strains DW5-4(T) and NH7I_ 1(T) was 99.8 %, and between each of the two strains and four type strains, B. pumilus ATCC 7061(T), Bacillus safensis FO-36b(T), Bacillus altitudinis 41KF2b(T) and Bacillus xiamenensis HYC-10(T), were above 99.4 %. The digital DNA-DNA hybridization value between the two novel strains was 41.6 %, and between each of the two strains and the four above-mentioned type strains were below 53.0 %. In both strains, the principal fatty acids were iso-C-15 : 0 and anteiso-C-15 : 0, and the isoprenoid quinone was identified as MK-7 (100 %). The DNA G+C contents of strains DW5-4(T) and NH7I_ 1(T) were 41.4 and 41.3 mol%, respectively. Based on phenotypic and genotypic characteristics, two novel species of the genus Bacillus are proposed: Bacillus zhangzhouensis sp. nov., with DW5-4(T) (5MCCC 1A08372(T)=LMG 27144(T)=KCTC 33531(T)) as the type strain, and Bacillus australimaris sp. nov., with NH7I_ 1(T) (=MCCC 1A05787(T)=LMG 27697(T)=KCTC 33532(T)) as the type strain.