Erythropoietin increases glutathione peroxidase enzyme activity and decreases lipid peroxidation levels in hypoxic-ischemic brain injury in neonatal rats

被引:90
作者
Kumral, A
Gonenc, S
Acikgoz, O
Sonmez, A
Genc, K
Yilmaz, O
Gokmen, N
Duman, N
Ozkan, H [1 ]
机构
[1] Dokuz Eylul Univ, Sch Med, Dept Pediat, TR-35340 Izmir, Turkey
[2] Dokuz Eylul Univ, Sch Med, Dept Physiol, TR-35340 Izmir, Turkey
[3] Dokuz Eylul Univ, Learning Resources Ctr Res Lab, TR-35340 Izmir, Turkey
[4] Dokuz Eylul Univ, Ctr Anim Res, TR-35340 Izmir, Turkey
[5] Dokuz Eylul Univ, Sch Med, Dept Anaesthesiol & Reanimat, TR-35340 Izmir, Turkey
来源
BIOLOGY OF THE NEONATE | 2005年 / 87卷 / 01期
关键词
erythropoietin; neonatal rat; hypoxic-ischemic brain injury; lipid peroxidation; oxidative stress; glutathione peroxidase;
D O I
10.1159/000080490
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Background: We have previously shown that erythropoietin (Epo) exerts neuroprotective effects in the Rice-Vannucci model of neonatal hypoxic-ischemic brain injury. However, the mechanisms of Epo protection in this model are still unclear. Objectives: In the present study, we studied the effects of systemically administered Epo on lipid peroxidation levels and antioxidant enzyme ( superoxide dismutase and glutathione peroxidase) activities following hypoxic-ischemic brain injury in neonatal rats. Methods: Seven-day-old Wistar rat pups were subjected to left carotid artery occlusion followed by 2.5 h of hypoxic exposure. Brain lipid peroxidation levels and antioxidant enzyme activities were measured in the injured hemispheres 24 h after the hypoxic-ischemic insult. Results: Hypoxic-ischemic injury significantly increased the thiobarbituric acid-reactive substance levels in the injured hemispheres as compared to the control group. In addition, glutathione peroxidase activity was significantly elevated in Epo-treated animals compared to saline-treated animals and the control group. Conclusions: These results suggest that Epo exerts neuroprotective effects against hypoxic-ischemic brain injury at least partially via the modulation of antioxidant enzyme activity. Copyright (C) 2005 S. Karger AG, Basel.
引用
收藏
页码:15 / 18
页数:4
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