The behavior of antibiotic-resistance genes and their relationships with the bacterial community and heavy metals during sewage sludge composting

被引:50
作者
Qiu, Xiuwen [1 ,2 ]
Zhou, Guixiang [1 ,2 ]
Wang, Huijuan [2 ]
Wu, Xiaoyu [3 ]
机构
[1] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China
[2] Jiujiang Univ, Jiangxi Prov Key Lab Ind Ecol Simulat & Environm, Jiujiang 332005, Peoples R China
[3] Jiangxi Agr Univ, Coll Biosci & Bioengn, Nanchang 330045, Peoples R China
基金
中国国家自然科学基金;
关键词
Sewage sludge; Composting; Antibiotic resistance genes; Bacterial community; Heavy metals; WASTE-WATER TREATMENT; MICROBIAL COMMUNITY; BIOCHAR INFLUENCES; FOOD WASTE; SOIL; PATHOGENS; FATE; PEAT;
D O I
10.1016/j.ecoenv.2021.112190
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The main contributors to antibiotic resistance genes (ARGs) profiles during the composting process under the addition of biochar (BC) and peat (PT) were not fully explored. This study investigated the influence of BC and PT amendment on ARGs fate, the bacterial community and heavy metals in sewage sludge compost. Compared to control, BC and PT declined the total abundances of ARGs by 17.6% and 43.0% after composting. Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria were the dominant phyla across the composting process, among which, members of Firmicutes (mainly Bacillaceae) were the potential hosts for multiple ARGs. BC and PT addition declined the abundance of bacterial pathogens such as Bacteroides and Pseudomonas. Besides, the concentrations of copper (Cu), zinc (Zn) and lead (Pb) were less in BC and PT treatments than control on day 40, and these metals displayed significant positive correlations to sul1 and intI1. Furthermore, variation partitioning analysis (VPA) revealed that the bacterial community exhibited the most contribution to the ARG patterns, as much as 34.0%, followed by heavy metals (10.8%) and intI1 gene (1.5%). These results suggested that biochar and peat can reduce the risks of ARGs in sewage sludge mainly by sharping the bacterial composition.
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页数:8
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