Biotransformation of betulin by Mucor subtilissimus to discover anti-inflammatory derivatives

被引：17
作者：
Li, Jianlin [1 ]
Jiang, Baocheng [1 ]
Chen, Chen [1 ]
Fan, Boyi [1 ]
Huang, Huilian [2 ]
Chen, Guangtong [1 ]
机构：
[1] Nantong Univ, Sch Pharm, 19 Qixiu Rd, Nantong 226001, Peoples R China
[2] Jiangxi Univ Tradit Chinese Med, Key Lab Modern Preparat TCM, Minist Educ, Nanchang, Jiangxi, Peoples R China
来源：
PHYTOCHEMISTRY | 2019年 / 166卷
基金：
中国国家自然科学基金;
关键词：
Mucor subtilissimus; Mucoraceae; Biotransformation; Betulin; Triterpene; Anti-inflammatory activity; ANTICANCER ACTIVITIES; ASPERGILLUS-NIGER; RACEMOSUS; 20(S)-PROTOPANAXATRIOL; 20(R)-PANAXATRIOL; INJURY; MICE; BARK;
D O I：
10.1016/j.phytochem.2019.112076
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Biotransformation of lupane-type triterpenoid betulin was carried out with Mucor subtilissimus CGMCC 3.2456. Yielded nine previously undescribed hydroxylated compounds. M. subtilissimus biotransformation provided C-7, C-11, C-15 and C-24 hydroxylated compounds along with C-7 oxidized and C-28 acetylated derivatives. The structures of the metabolites were established based on extensive NMR and FIR-ESI-MS data analyses. Furthermore, we found that most of the metabolites exhibited pronounced inhibitory activities on lipopoly-saccharides-induced NO production in RAW264.7 cells.
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页数：6
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