Genome-wide SWAp-Tag yeast libraries for proteome exploration

被引：107

作者：

Weill, Uri ^{[1
]}

Yofe, Ido ^{[1
]}

Sass, Ehud ^{[2
]}

Stynen, Bram ^{[3
]}

Davidi, Dan ^{[4
]}

Natarajan, Janani ^{[5
]}

Ben-Menachem, Reut ^{[6
]}

Avihou, Zohar ^{[1
]}

Goldman, Omer ^{[1
]}

Harpaz, Nofar ^{[1
]}

Chuartzman, Silvia ^{[1
]}

Kniazev, Kiril ^{[1
]}

Knoblach, Barbara ^{[7
]}

Laborenz, Janina ^{[8
]}

Boos, Felix ^{[8
]}

Kowarzyk, Jacqueline ^{[3
]}

Ben-Dor, Shifra ^{[9
]}

Zalckvar, Einat ^{[1
]}

Herrmann, Johannes M. ^{[8
]}

Rachubinski, Richard A. ^{[7
]}

Pines, Ophry ^{[6
]}

Rapaport, Doron ^{[5
]}

Michnick, Stephen W. ^{[3
]}

Levy, Emmanuel D. ^{[2
]}

Schuldiner, Maya ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Mol Genet, Rehovot, Israel

[2] Weizmann Inst Sci, Dept Biol Struct, Rehovot, Israel

[3] Univ Montreal, Fac Med, Dept Biochim, Montreal, PQ, Canada

[4] Weizmann Inst Sci, Dept Plant & Environm Sci, Rehovot, Israel

[5] Univ Tubingen, Interfac Inst Biochem, Tubingen, Germany

[6] Hebrew Univ Jerusalem, Dept Microbiol & Mol Genet, IMRIC, Fac Med, Jerusalem, Israel

[7] Univ Alberta, Dept Cell Biol, Edmonton, AB, Canada

[8] Univ Kaiserslautern, Dept Cell Biol, Kaiserslautern, Germany

[9] Weizmann Inst Sci, Dept Life Sci Core Facil, Rehovot, Israel

来源：

NATURE METHODS | 2018年 / 15卷 / 08期

基金：

以色列科学基金会; 加拿大健康研究院; 欧洲研究理事会;

关键词：

SACCHAROMYCES-CEREVISIAE; IN-VIVO; TRANSMEMBRANE TOPOLOGY; GENE OVEREXPRESSION; FLUORESCENT PROTEIN; GLOBAL ANALYSIS; BUDDING YEAST; MITOCHONDRIA; EXPRESSION; PREDICTION;

D O I：

10.1038/s41592-018-0044-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of similar to 5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

引用

页码：617 / +

页数：13

共 56 条

[1] Tom70 enhances mitochondrial preprotein import efficiency by binding to internal targeting sequences [J].