Cellulase Hyperproducing Mutants Derived from the Fungus Trichoderma reesei QM9414 Produced Large Amounts of Cellulase at the Enzymatic and Transcriptional Levels

被引:21
作者
Fujii, Tatsuya [1 ]
Murakami, Katsuji [1 ]
Sawayama, Shigeki [1 ]
机构
[1] Natl Inst Adv Ind Sci & Technol, Biomass Technol Res Ctr, Hiroshima 7370197, Japan
关键词
cellulase; Trichoderma reesei; cellobiohydrolase I; endoglucanase I; L-SORBOSE; ETHANOL; BIOMASSES;
D O I
10.1271/bbb.90655
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cellulase hyperproducing mutants derived from the fungus Trichoderma reesei QM9414 were analyzed. They exhibited higher filter-paper degrading activity and a lower growth rate than the wild-type QM9414 strain. Transcription of the cellobiohydrolase I and endoglucanase I genes in the mutants was also greater than that of QM9414, suggesting that cellulase hyperproduction by these mutants was regulated at the transcriptional level.
引用
收藏
页码:419 / 422
页数:4
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