Cellulase Hyperproducing Mutants Derived from the Fungus Trichoderma reesei QM9414 Produced Large Amounts of Cellulase at the Enzymatic and Transcriptional Levels

被引:21
作者
Fujii, Tatsuya [1 ]
Murakami, Katsuji [1 ]
Sawayama, Shigeki [1 ]
机构
[1] Natl Inst Adv Ind Sci & Technol, Biomass Technol Res Ctr, Hiroshima 7370197, Japan
关键词
cellulase; Trichoderma reesei; cellobiohydrolase I; endoglucanase I; L-SORBOSE; ETHANOL; BIOMASSES;
D O I
10.1271/bbb.90655
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cellulase hyperproducing mutants derived from the fungus Trichoderma reesei QM9414 were analyzed. They exhibited higher filter-paper degrading activity and a lower growth rate than the wild-type QM9414 strain. Transcription of the cellobiohydrolase I and endoglucanase I genes in the mutants was also greater than that of QM9414, suggesting that cellulase hyperproduction by these mutants was regulated at the transcriptional level.
引用
收藏
页码:419 / 422
页数:4
相关论文
共 21 条
[1]   ATP MEASUREMENT FOR CELLULASE PRODUCTION CONTROL [J].
COCHET, N ;
TYAGI, RD ;
GHOSE, TK ;
LEBEAULT, JM .
BIOTECHNOLOGY LETTERS, 1984, 6 (03) :155-160
[2]   Global carbon utilization profiles of wild-type, mutant, and transformant strains of Hypocrea jecorina [J].
Druzhinina, IS ;
Schmoll, M ;
Seiboth, B ;
Kubicek, CP .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (03) :2126-2133
[3]   Crystallization behaviour of new poly(tetramethyleneterephthalamide) nanocomposites containing SiO2 fillers with distinct morphologies [J].
Esteves, ACC ;
Barros-Timmons, AM ;
Martins, JA ;
Zhang, W ;
Cruz-Pinto, J ;
Trindade, T .
COMPOSITES PART B-ENGINEERING, 2005, 36 (01) :51-59
[4]   Lactose Enhances Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus [J].
Fang, Xu ;
Yano, Shinichi ;
Inoue, Hiroyuki ;
Sawayama, Shigeki .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2008, 106 (02) :115-120
[5]   Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei [J].
Foreman, PK ;
Brown, D ;
Dankmeyer, L ;
Dean, R ;
Diener, S ;
Dunn-Coleman, NS ;
Goedegebuur, F ;
Houfek, TD ;
England, GJ ;
Kelley, AS ;
Meerman, HJ ;
Mitchell, T ;
Mitchinson, C ;
Olivares, HA ;
Teunissen, PJM ;
Yao, J ;
Ward, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (34) :31988-31997
[6]   CHARACTERISTICS OF FUNGAL CELLULASES [J].
GOYAL, A ;
GHOSH, B ;
EVELEIGH, D .
BIORESOURCE TECHNOLOGY, 1991, 36 (01) :37-50
[7]  
Gritzali M., 1979, Adv. Chem. Ser, V181, P237, DOI DOI 10.1021/BA-1979-0181.CH012
[8]  
Ilmen M, 1997, APPL ENVIRON MICROB, V63, P1298
[9]   Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus [J].
Inoue, Hiroyuki ;
Yano, Shinichi ;
Endo, Takashi ;
Sakaki, Tsuyoshi ;
Sawayama, Shigeki .
BIOTECHNOLOGY FOR BIOFUELS, 2008, 1 (1)
[10]   PRODUCTION OF ETHANOL FROM BIOMASSES .2. PREPARATION OF MUTANTS RESISTANT TO CATABOLITE REPRESSION OF TRICHODERMA-REESEI [J].
KAWAMORI, M ;
MORIKAWA, Y ;
SHINSHA, Y ;
TAKAYAMA, K ;
TAKASAWA, S .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, 49 (10) :2875-2879