Coaxial Alginate Hydrogels: From Self-Assembled 3D Cellular Constructs to Long-Term Storage

被引:18
作者
Gryshkov, Oleksandr [1 ,2 ]
Mutsenko, Vitalii [1 ,2 ]
Tarusin, Dmytro [3 ]
Khayyat, Diaa [1 ,2 ]
Naujok, Ortwin [4 ]
Riabchenko, Ekaterina [5 ]
Nemirovska, Yuliia [3 ]
Danilov, Arseny [5 ]
Petrenko, Alexander Y. [3 ]
Glasmacher, Birgit [1 ,2 ]
机构
[1] Leibniz Univ Hannover, Inst Multiphase Proc, Univ 1,Bldg 8143, D-30823 Hannover, Germany
[2] Lower Saxony Ctr Biomed Engn Implant Res & Dev, Stadtfelddamm 34, D-30625 Hannover, Germany
[3] Natl Acad Sci Ukraine, Inst Problems Cryobiol & Cryomed, 23 Pereyaslavsky St, UA-61015 Kharkiv, Ukraine
[4] Hannover Med Sch, Inst Clin Biochem, Carl Neuberg Str 1, D-30625 Hannover, Germany
[5] Natl Res Univ Elect Technol, Inst Biomed Syst, Moscow 124498, Russia
关键词
cell encapsulation; coaxial electrospraying; tissue cryopreservation; core-shell capsules; scaffolds; cellular structures; multipotent stromal cells; thermomechanical stress; RAMAN spectroscopy; swelling; MULTIPOTENT STROMAL CELLS; STEM-CELLS; CALLITHRIX-JACCHUS; RAMAN-SPECTROSCOPY; ICE NUCLEATION; CRYOPRESERVATION; ENCAPSULATION; BEADS; ACID; DIFFERENTIATION;
D O I
10.3390/ijms22063096
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alginate as a versatile naturally occurring biomaterial has found widespread use in the biomedical field due to its unique features such as biocompatibility and biodegradability. The ability of its semipermeable hydrogels to provide a favourable microenvironment for clinically relevant cells made alginate encapsulation a leading technology for immunoisolation, 3D culture, cryopreservation as well as cell and drug delivery. The aim of this work is the evaluation of structural properties and swelling behaviour of the core-shell capsules for the encapsulation of multipotent stromal cells (MSCs), their 3D culture and cryopreservation using slow freezing. The cells were encapsulated in core-shell capsules using coaxial electrospraying, cultured for 35 days and cryopreserved. Cell viability, metabolic activity and cell-cell interactions were analysed. Cryopreservation of MSCs-laden core-shell capsules was performed according to parameters pre-selected on cell-free capsules. The results suggest that core-shell capsules produced from the low viscosity high-G alginate are superior to high-M ones in terms of stability during in vitro culture, as well as to solid beads in terms of promoting formation of viable self-assembled cellular structures and maintenance of MSCs functionality on a long-term basis. The application of 0.3 M sucrose demonstrated a beneficial effect on the integrity of capsules and viability of formed 3D cell assemblies, as compared to 10% dimethyl sulfoxide (DMSO) alone. The proposed workflow from the preparation of core-shell capsules with self-assembled cellular structures to the cryopreservation appears to be a promising strategy for their off-the-shelf availability.
引用
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页码:1 / 31
页数:31
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