A new monoclonal antibody for the sensitive detection of cyanazine and other s-triazines in water by ELISA

被引：13
作者：
Bruun, L
Koch, C
Jakobsen, MH
Aamand, J
机构：
[1] Geol Survey Denmark & Greenland, Dept Geochem, DK-2400 Copenhagen NV, Denmark
[2] Statens Serum Inst, Dept Appl Immunol, DK-2300 Copenhagen, Denmark
[3] Exiqon AS, DK-2950 Vedboek, Denmark
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2000年 / 12卷 / 04期
关键词：
monoclonal antibody; cyanazine; covalent immobilization; immunoassay; herbicide; pesticide;
D O I：
10.1080/09540100020008128
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A new monoclonal antibody with specificity for s-triazine herbicides was prepared using a tertbutyl-s-triazine hapten. An assay was developed, employing covalently immobilized hapten and monoclonal antibody labeled with horseradish peroxidase. This assay system had a detection limit of 0.01 mu gl(-1) cyanazine, with a coefficient of variation less than 3%. Analysis on three different spiked water types showed an average recovery of 101% at 0.1 mu gl(-1) cyanazine. Cross-reactions were found for atrazine, terbuthylazine and propazine in the range 18-62%. This immunoassay is therefore capable of detecting four different s-triazine herbicides at concentrations well below the limit value for drinking water of 0.1 mu gl(-1) stipulated by the EEC Council Directive on drinking water.
引用
收藏
页码：253 / 262
页数：10
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