The expression and significance of IDH1 and p53 in osteosarcoma

被引：27
作者：
Hu, Xiang [1 ]
Yu, Ai-Xi [1 ]
Qi, Bai-Wen [1 ]
Fu, Tao [1 ]
Wu, Gang [1 ]
Zhou, Min [1 ]
Luo, Jun [2 ]
Xu, Jun-Hua [1 ]
机构：
[1] Wuhan Univ, Zhongnan Hosp, Dept Orthoped, Wuhan 430071, Peoples R China
[2] Wuhan Univ, Zhongnan Hosp, Dept Pathol, Wuhan 430071, Peoples R China
来源：
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH | 2010年 / 29卷
关键词：
PRIMARY OSTEOGENIC-SARCOMA; EN-BLOC RESECTION; IMMUNOHISTOCHEMICAL EXPRESSION; MUTATIONS; GENE; BONE; DNA; CHEMOTHERAPY; PTEN; DEHYDROGENASE;
D O I：
10.1186/1756-9966-29-43
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: To detect the expression of isocitrate dehydrogenase 1 (IDH1) and transformation-related protein 53 (p53) in osteosarcoma and analyze the correlation between them and the clinico-pathological features. Methods: The expressions of IDH1 and p53 were detected in human osteosarcoma cell lines (MG-63 and U2OS) by immunocytochemistry, Real-time PCR and Western Blotting. The expressions of IDH1 and p53 in formalin-fixed paraffin-embedded tissue sections from 44 osteosarcoma patients were determined by immunohistochemistry, and the correlation between them and clinicopagthological features were analyzed. None of these patients received chemotherapy prior to surgery. Results: IDH1 is detected in osteosarcoma cell lines and biopsies. IDH1 expresses higher in U2OS cells with wild type p53 than in MG-63 cells with mutation p53. IDH1 correlates with histological Rosen grade and metastasis negatively. P53 correlates with histological Rosen grade, metastasis and overall survival in clinical osteosarcoma biopsies. Osteosarcoma patients with High IDH1 expression have a very high p53 expression. Conclusion: IDH1 may correlate with p53 and be a candidate biomarker for osteosarcoma correlate with histological Rosen grade and metastasis.
引用
收藏
页数：10
相关论文
共 54 条
  • [1] CHROMOSOME-17 DELETIONS AND P53 GENE-MUTATIONS IN COLORECTAL CARCINOMAS
    BAKER, SJ
    FEARON, ER
    NIGRO, JM
    HAMILTON, SR
    PREISINGER, AC
    JESSUP, JM
    VANTUINEN, P
    LEDBETTER, DH
    BARKER, DF
    NAKAMURA, Y
    WHITE, R
    VOGELSTEIN, B
    [J]. SCIENCE, 1989, 244 (4901) : 217 - 221
  • [2] p53 isoforms can regulate p53 transcriptional activity
    Bourdon, JC
    Fernandes, K
    Murray-Zmijewski, F
    Liu, G
    Diot, A
    Xirodimas, DP
    Saville, MK
    Lane, DP
    [J]. GENES & DEVELOPMENT, 2005, 19 (18) : 2122 - 2137
  • [3] New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase AKT pathway
    Cantley, LC
    Neel, BG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (08) : 4240 - 4245
  • [4] INACTIVATION OF P53 GENE IN HUMAN AND MURINE OSTEOSARCOMA CELLS
    CHANDAR, N
    BILLIG, B
    MCMASTER, J
    NOVAK, J
    [J]. BRITISH JOURNAL OF CANCER, 1992, 65 (02) : 208 - 214
  • [5] P53 SWEEPS THROUGH CANCER-RESEARCH
    CULOTTA, E
    KOSHLAND, DE
    [J]. SCIENCE, 1993, 262 (5142) : 1958 - 1961
  • [6] The multiple roles of PTEN in tumor suppression
    Di Cristofano, A
    Pandolfi, PP
    [J]. CELL, 2000, 100 (04) : 387 - 390
  • [7] Current treatment of osteosarcoma
    Ferguson, WS
    Goorin, AM
    [J]. CANCER INVESTIGATION, 2001, 19 (03) : 292 - 315
  • [8] Copy number gains in EGFR and copy number losses in PTEN are common events in osteosarcoma tumors
    Freeman, Serena S.
    Allen, Steven W.
    Ganti, Ramapriya
    Wu, Jianrong
    Ma, Jing
    Su, Xiaoping
    Neale, Geoff
    Dome, Jeffrey S.
    Daw, Najat C.
    Khoury, Joseph D.
    [J]. CANCER, 2008, 113 (06) : 1453 - 1461
  • [9] Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumours
    Graeber, TG
    Osmanian, C
    Jacks, T
    Housman, DE
    Koch, CJ
    Lowe, SW
    Giaccia, AJ
    [J]. NATURE, 1996, 379 (6560) : 88 - 91
  • [10] The PTEN/PI3K pathway governs normal vascular development and tumor angiogenesis
    Hamada, K
    Sasaki, T
    Koni, PA
    Natsui, M
    Kishimoto, H
    Sasaki, J
    Yajima, N
    Horie, Y
    Hasegawa, G
    Naito, M
    Miyazaki, J
    Suda, T
    Itoh, H
    Nakao, K
    Mak, TW
    Nakano, T
    Suzuki, A
    [J]. GENES & DEVELOPMENT, 2005, 19 (17) : 2054 - 2065
123456