Regulation of VEGFA, KRAS, and NFE2L2 Oncogenes by MicroRNAs in Head and Neck Cancer

被引：9

作者：

Cuzziol, Caroline Izak ^{[1
]}

Marzochi, Ludimila Leite ^{[1
]}

Possebon, Vitoria Scavacini ^{[2
]}

Kawasaki-Oyama, Rosa Sayoko ^{[1
]}

Mattos, Marlon Fraga ^{[1
]}

Serafim Junior, Vilson ^{[2
]}

Muniz Ferreira, Leticia Antunes ^{[1
]}

Pavarino, Erika Cristina ^{[1
]}

Urbanin Castanhole-Nunes, Marcia Maria ^{[1
]}

Goloni-Bertollo, Eny Maria ^{[1
]}

机构：

[1] Fac Med Sao Jose do Rio Preto FAMERP, Dept Mol Biol, Res Unit Genet & Mol Biol UPGEM, BR-15090000 Sao Jose Do Rio Preto, Brazil

[2] Sao Paulo State Univ Unesp, Inst Biosci Humanities & Exact Sci, Campus Sao Jose Rio Preto, BR-15054000 Sao Jose Do Rio Preto, Brazil

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 13期

基金：

巴西圣保罗研究基金会; 瑞典研究理事会;

关键词：

miR-17-5p; miR-140-5p; miR-874-3p; angiogenesis; ANGIOGENESIS; PROGRESSION; EXPRESSION; CARCINOMA; NRF2;

D O I：

10.3390/ijms23137483

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mutations and alterations in the expression of VEGFA, KRAS, and NFE2L2 oncogenes play a key role in cancer initiation and progression. These genes are enrolled not only in cell proliferation control, but also in angiogenesis, drug resistance, metastasis, and survival of tumor cells. MicroRNAs (miRNAs) are small, non-coding regulatory RNA molecules that can regulate post-transcriptional expression of multiple target genes. We aimed to investigate if miRNAs hsa-miR-17-5p, hsa-miR-140-5p, and hsa-miR-874-3p could interfere in VEGFA, KRAS, and NFE2L2 expression in cell lines derived from head and neck cancer (HNC). FADU (pharyngeal cancer) and HN13 (oral cavity cancer) cell lines were transfected with miR-17-5p, miR-140-5p, and miR-874-3p microRNA mimics. RNA and protein expression analyses revealed that miR-17-5p, miR-140-5p and miR-874-3p overexpression led to a downregulation of VEGFA, KRAS, and NFE2L2 gene expression in both cell lines analyzed. Taken together, our results provide evidence for the establishment of new biomarkers in the diagnosis and treatment of HNC.

引用

页数：12

共 53 条

[51]

Yuan RB, 2018, EUR REV MED PHARMACO, V22, P7265, DOI 10.26355/eurrev_201811_16261

[52]

Zhang JR, 2018, EUR REV MED PHARMACO, V22, P5994, DOI 10.26355/eurrev_201809_15934

[53] Exposure to tris(1,3-dichloro-2-propyl) phosphate (TDCPP) induces vascular toxicity through Nrf2-VEGF pathway in zebrafish and human umbilical vein endothelial cells [J].

Zhong, Xiali ;

Qiu, Jiahuang ;

Kang, Jianmeng ;

Xing, Xiumei ;

Shi, Xiongjie ;

Wei, Yanhong .

ENVIRONMENTAL POLLUTION, 2019, 247 :293-301

← 1 2 3 4 5 6 →