In Vivo Cancer Cells Elimination Guided by Aptamer-Functionalized Gold-Coated Magnetic Nanoparticles and Controlled with Low Frequency Alternating Magnetic Field

被引：48

作者：

Belyanina, Irina V. ^{[1
,3
]}

Zamay, Tatiana N. ^{[1
,3
]}

Zamay, Galina S. ^{[1
,2
]}

Zamay, Sergey S. ^{[2
]}

Kolovskaya, Olga S. ^{[1
,2
]}

Ivanchenko, Tatiana I. ^{[1
]}

Denisenko, Valery V. ^{[3
,5
]}

Kirichenko, Andrey K. ^{[1
]}

Glazyrin, Yury E. ^{[1
,2
]}

Garanzha, Irina V. ^{[1
,3
]}

Grigorieva, Valentina V. ^{[1
,3
]}

Shabanov, Alexandr V. ^{[2
]}

Veprintsev, Dmitry V. ^{[1
,2
]}

Sokolov, Alexey E. ^{[2
,3
]}

Sadovskii, Vladimir M. ^{[3
,5
]}

Gargaun, Ana ^{[4
]}

Berezovski, Maxim V. ^{[4
]}

Kichkailo, Anna S. ^{[1
,2
]}

机构：

[1] Krasnoyarsk State Med Univ, Krasnoyarsk, Russia

[2] Russian Acad Sci, KSC Siberian Branch, Fed Res Ctr, Krasnoyarsk, Russia

[3] Siberian Fed Univ, Krasnoyarsk, Russia

[4] Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON, Canada

[5] Inst Computat Modeling RAS SB, Krasnoyarsk, Russia

来源：

THERANOSTICS | 2017年 / 7卷 / 13期

基金：

俄罗斯科学基金会;

关键词：

cancer therapy; gold coated magnetic nanoparticles; DNA aptamers; low frequency alternating magnetic field; fibronectin; integrin; apoptosis; necrosis; PHOTOTHERMAL THERAPY; FIBRONECTIN; STIMULATION; INTEGRINS; APOPTOSIS; ADHESION; GROWTH;

D O I：

10.7150/thno.17089

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Biomedical applications of magnetic nanoparticles under the influence of a magnetic field have been proved useful beyond expectations in cancer therapy. Magnetic nanoparticles are effective heat mediators, drug nanocarriers, and contrast agents; various strategies have been suggested to selectively target tumor cancer cells. Our study presents magnetodynamic nanotherapy using DNA aptamer-functionalized 50 nm gold-coated magnetic nanoparticles exposed to a low frequency alternating magnetic field for selective elimination of tumor cells in vivo. The cell specific DNA aptamer AS-14 binds to the fibronectin protein in Ehrlich carcinoma hence helps deliver the gold-coated magnetic nanoparticles to the mouse tumor. Applying an alternating magnetic field of 50 Hz at the tumor site causes the nanoparticles to oscillate and pull the fibronectin proteins and integrins to the surface of the cell membrane. This results in apoptosis followed by necrosis of tumor cells without heating the tumor, adjacent healthy cells and tissues. The aptamer-guided nanoparticles and the low frequency alternating magnetic field demonstrates a unique non-invasive nanoscalpel technology for precise cancer surgery at the single cell level.

引用

页码：3326 / 3337

页数：12

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