Purification of the isoflavonoid puerarin by adsorption chromatography on cross-linked 12% agarose

被引：33

作者：

He, XL

Tan, TW

Janson, JC

机构：

[1] Uppsala Univ, Uppsala Biomed Ctr, Dept Surface Biotechnol, SE-75123 Uppsala, Sweden

[2] Beijing Univ Chem Technol, Sch Life Sci & Technol, Dept Biotechnol, Beijing 100029, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1057卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

isoflavonoid separation; agarose cross-linker; puerarin; acetic acid quenching;

D O I：

10.1016/j.chroma.2004.09.068

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The isoflavonoid puerarin in extracts of the well-known traditional Chinese drug Radix puerariae (root of the plant Pueraria lobata) can be separated from other isoflavonoids by adsorption chromatography on the cross-linked 12% agarose gel Superose 12 equilibrated in distilled water. The adsorption is totally quenched by the addition of 50% acetic acid. The separation of the isoflavonoids is tentatively ascribed to interaction with the residues of the cross-linking reagents used in the manufacturing process of Superose 12. Thus, no useful separation can be achieved with non-cross-linked 12% agarose gel media. Symmetric elution profiles at high sample loadings (16 mg on a 24 ml column) suggest linear adsorption isotherms for the isoflavonoids. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：95 / 100

页数：6