Clinical Origin and Species Distribution of Fusarium spp. Isolates Identified by Molecular Sequencing and Mass Spectrometry: A European Multicenter Hospital Prospective Study

被引：11

作者：

Normand, Anne-Cecile ^{[1
]}

Imbert, Sebastien ^{[1
,2
,3
]}

Brun, Sophie ^{[2
]}

Al-Hatmi, Abdullah M. S. ^{[3
,4
]}

Chryssanthou, Erja ^{[5
]}

Cassaing, Sophie ^{[6
]}

Schuttler, Christine ^{[7
]}

Hasseine, Lilia ^{[8
]}

Mahinc, Caroline ^{[9
]}

Costa, Damien ^{[10
]}

Bonnal, Christine ^{[11
]}

Ranque, Stephane ^{[12
]}

Sautour, Marc ^{[13
]}

Rubio, Elisa ^{[14
]}

Delhaes, Laurence ^{[15
]}

Riat, Arnaud ^{[16
]}

Sendid, Boualem ^{[17
]}

Kristensen, Lise ^{[18
]}

Brandenberger, Marcel ^{[19
]}

Guitard, Juliette ^{[20
]}

Packeu, Ann ^{[21
]}

Piarroux, Renaud ^{[1
,22
]}

Fekkar, Arnaud ^{[1
,23
]}

机构：

[1] Grp Hosp La Pitie Salpetriere, AP HP, Serv Parasitol Mycol, F-75013 Paris, France

[2] Hop Avicenne, AP HP, Serv Parasitol Mycol, F-93000 Bobigny, France

[3] Radboud Univ Nijmegen, Canisius Wilhelmina Hosp, Med Ctr, Ctr Expertise Mycol, NL-6525 Nijmegen, Netherlands

[4] Univ Nizwa, Dept Microbiol, Nat & Med Sci Res Ctr, Nizwa 616, Oman

[5] Karolinska Inst, Dept Lab Med, Div Clin Microbiol, S-17177 Stockholm, Sweden

[6] CHU Toulouse, Serv Parasitol Mycol, F-31000 Toulouse, France

[7] Lab Biogrp LCD, F-92300 Levallois Perret, France

[8] CHU Nice, Serv Parasitol Mycol, F-06200 Nice, France

[9] CHU St Etienne, Serv Parasitol Mycol, F-42000 St Etienne, France

[10] Ctr Hosp Univ Rouen, Serv Parasitol Mycol, F-76000 Rouen, France

[11] Hop Bichat Claude Bernard, AP HP, Serv Parasitol Mycol, F-75018 Paris, France

[12] Aix Marseille Univ, AP HM, IRD, SSA,VITROME,IHU Mediterranee Infect, F-13005 Marseille, France

[13] CHU Dijon, Serv Parasitol Mycol, F-21079 Dijon, France

[14] Univ Barcelona, ISGlobal Barcelona Inst Global Hlth, Hosp Clin, Dept Microbiol,Spain CDB, Barcelona 08036, Spain

[15] CHU Bordeaux, Grp Hosp Pellegrin, Serv Mycol, F-33404 Bordeaux, France

[16] Univ Hosp Geneva, Div Lab Med, Lab Bacteriol, Rue Gabrielle Perret Gentil 4, CH-1205 Geneva, Switzerland

[17] CHU Lille, Dept Parasitol Mycol, F-59000 Lille, France

[18] Aarhus Univ Hosp, Dept Clin Microbiol, DK-8200 Aarhus, Denmark

[19] Labor Synlab Luzern, CH-6002 Luzern, Switzerland

[20] Sorbonne Univ, Hop St Antoine, AP HP, INSERM,Ctr Rech St Antoine,CRSA,Serv Parasitol My, F-75012 Paris, France

[21] Sciensano, BCCM IHEM Collect, Mycol & Aerobiol Unit, B-1000 Brussels, Belgium

[22] Sorbonne Univ, Inst Pierre Louis Epidemiol & Sante Publ, INSERM, F-75571 Paris, France

[23] Sorbonne Univ, Cimi Paris, CNRS, INSERM,Ctr Immunol & Malad Infect, F-75005 Paris, France

来源：

JOURNAL OF FUNGI | 2021年 / 7卷 / 04期

关键词：

fusariosis; mALDI ToF mass spectrometry; DNA sequencing; elongation factor; Fusarium species complex;

D O I：

10.3390/jof7040246

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fusarium spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of Fusarium spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify Fusarium isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any Fusarium isolates encountered either in human pathology or in the environment.

引用

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