The role of chromophore in the lipid-protein interactions in bacteriorhodopsin-phosphatidylcholine vesicles

被引：3
作者：
Bryl, K [1 ]
Yoshihara, K
机构：
[1] Univ Warmia & Mazury, Dept Phys & Biophys, PL-10957 Olsztyn, Poland
[2] Suntory Inst Bioorgan Res, SUNBOR, Osaka 618, Japan
来源：
FEBS LETTERS | 2000年 / 480卷 / 2-3期
关键词：
lipid-protein interaction; chromophore; bacteriorhodopsin; lipid phase transition; fluorescence excitation spectrum;
D O I：
10.1016/S0014-5793(00)01910-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
By fluorescence and phase properties of a 1-acyl-2-[8-(2-anthroyl)-octanoyl]-sn-glycero-3-phosphocholine probe, the influence of the chromophore on the phase transition of bacteriorhodopsin-lipid vesicles was investigated. It was observed that removal of the chromophore led to the down-shifting of the phase transition temperatures. The temperatures corresponding to the beginning and ending of the gel-liquid phase transition mere also influenced. This demonstrated that the liquid phase is reached more easily when the chromophore is bleached. The results indicate that removal of the chromophore alters the protein-lipid interactions. It is suggested that this alteration might he related to the change in the lipid molecular packing. (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B,V. All rights reserved.
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收藏
页码：123 / 126
页数：4
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