Preparation of 4-vinylphenol using pHCA decarboxylase in a two-solvent medium

被引：22

作者：

Ben-Bassat, Arie ^{[1
]}

Breinig, Sabine ^{[1
]}

Crum, Grace A. ^{[1
]}

Huang, Lixuan ^{[1
]}

Altenbaugh, Amee La Bonte ^{[1
]}

Rizzo, Nancy ^{[1
]}

Trotman, Robert J. ^{[1
]}

Vannelli, Todd ^{[1
]}

Sariaslani, F. Sima ^{[1
]}

Haynie, Sharon L. ^{[1
]}

机构：

[1] EI du Pont Nemours & Co Expt Stn, Cent Res & Dev Dept, Wilmington, DE 19880 USA

来源：

ORGANIC PROCESS RESEARCH & DEVELOPMENT | 2007年 / 11卷 / 02期

关键词：

D O I：

10.1021/op0602472

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An efficient synthesis of 4-vinylphenol (4-VP) from para-hydroxycinnamic acid (pHCA) that uses the enzyme para-hydroxycinnamic acid decarboxylase (PDC) in a two-phase aqueous-organic solvent has been developed. The 4-VP titer and catalyst productivity (17 g L-1; 1010 g 4-VP isolated g(-1) catalyst) greatly exceed those for intact cells expressing PDC or calcium alginate bead-immobilized PDC-expressing cells in an aqueous buffer (5 g L-1; 4.4 to 64 g of isolated 4-VP per gram of catalyst). In this two-phase reaction design, the organic solvent (e.g., toluene) enabled continuous extraction of 4-VP into the organic phase and either its recovery as a crystalline solid with low color or its direct conversion to 4-acetoxystyrene.

引用

页码：278 / 285

页数：8

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