Hypotonic stress-induced NO production in endothelium depends on endogenous ATP

被引：34

作者：

Kimura, C ^{[1
]}

Koyama, T ^{[1
]}

Oike, M ^{[1
]}

Ito, Y ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Dept Pharmacol, Fukuoka 8128582, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 274卷 / 03期

关键词：

hypotonic stress; ATP; nitric oxide; endothelial cell;

D O I：

10.1006/bbrc.2000.3205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism by which mechanical stress induces nitric oxide (NO) synthesis in endothelium is still controversial. Hypotonic stress (HTS, -20%) induced ATP release, which evoked Ca2+ transients in bovine aortic endothelial cells (BAEC), HTS also induced NO synthesis, assessed by DAF-S fluorescence, which was suppressed by inhibiting endogenous ATP-induced Ca2+ transients with suramin or neomycin, Exogenously applied ATP mimicked these responses. Pretreatment with wortmannin did not affect DAF-2 fluorescence, suggesting that Akt phosphorylation was not involved in MTS-induced NO synthesis, These results indicate that endogenous ATP plays a central role in HTS-induced NO synthesis in BAEC. (C) 2000 Academic Press.

引用

页码：736 / 740

页数：5

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