Exocytotic Vesicle Behaviour Assessed by Total Internal Reflection Fluorescence Microscopy

被引：48
作者：
Burchfield, James G. [1 ]
Lopez, Jamie A. [1 ]
Mele, Katarina [1 ,2 ]
Vallotton, Pascal [2 ]
Hughes, William E. [1 ,3 ]
机构：
[1] Garvan Inst Med Res, Sydney, NSW 2010, Australia
[2] CSIRO, Math & Informat Sci, N Ryde, NSW 1670, Australia
[3] St Vincents Hosp, Dept Med, Sydney, NSW 2010, Australia
来源：
TRAFFIC | 2010年 / 11卷 / 04期
关键词：
MIN6; BETA-CELLS; EVANESCENT-WAVE MICROSCOPY; SINGLE SECRETORY GRANULES; LIVE CHROMAFFIN CELLS; PLASMA-MEMBRANE; GLUCOSE-TRANSPORTER; GLUT4; TRAFFICKING; INSULIN EXOCYTOSIS; ACTIN CORTEX; DOCKING STEP;
D O I：
10.1111/j.1600-0854.2010.01039.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The regulated trafficking or exocytosis of cargo-containing vesicles to the cell surface is fundamental to all cells. By coupling the technology of fluorescently tagged fusion proteins with total internal reflection fluorescence microscopy (TIRFM), it is possible to achieve the high spatio-temporal resolution required to study the dynamics of sub-plasma membrane vesicle trafficking and exocytosis. TIRFM has been used in a number of cell types to visualize and dissect the various steps of exocytosis revealing how molecules identified via genetic and/or biochemical approaches are involved in the regulation of this process. Here, we summarize the contribution of TIRFM to our understanding of the mechanism of exocytosis and discuss the novel methods of analysis that are required to exploit the large volumes of data that can be produced using this technique.
引用
收藏
页码：429 / 439
页数：11
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