Effects and mechanisms of aloperine on 2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice

被引：67

作者：

Yuan, Xiao-Ying ^{[1
,2
]}

Liu, Wei ^{[1
,2
]}

Zhang, Ping ^{[1
]}

Wang, Rui-Yan ^{[1
]}

Guo, Jian-You ^{[3
]}

机构：

[1] Gen Hosp Air Force, Dept Dermatol, Beijing 1000142, Peoples R China

[2] China Med Univ, Hosp 1, Dept Dermatol, Shenyang 110001, Peoples R China

[3] Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 2010年 / 629卷 / 1-3期

基金：

中国国家自然科学基金;

关键词：

Aloperine; Allergic contact dermatitis; Tumor necrosis factor-alpha; Interleukin-1; beta; Interleukin-6; TUMOR-NECROSIS-FACTOR; FACTOR-ALPHA; SCRATCHING BEHAVIOR; SIGNALING PATHWAYS; LANGERHANS CELLS; DENDRITIC CELLS; SKIN; HYPERSENSITIVITY; EXPRESSION; CYTOKINES;

D O I：

10.1016/j.ejphar.2009.12.007

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Allergic contact dermatitis is a prototypic T-cell-mediated cutaneous inflammatory response. Multiple cell types, inflammatory mediators and cytokines are involved in the regulation of immunologic and inflammatory processes in allergic contact dermatitis. Aloperine is an isolated alkaloid found in the plant of Sophora alopecuroides L. It has been clinically proved effective in China for a long time for skin inflammatory diseases such as allergic contact dermatitis. However, the mechanism of aloperine on allergic contact dermatitis is largely unknown. Therefore, the aim of this study was to investigate the effect of aloperine on 2, 4-dinitrofluorobenzene (DNFB)-induced allergic contact dermatitis in BALB/c mice and the possible underlying mechanisms. The results showed that topical application of DNFB on the car provoked typical allergic contact dermatitis with ear swelling and ear erythema in BALB/c mice. Treatments with 1% aloperine suppressed DNFB-induced increase in ear thickness and ear erythema. Moreover, 1% aloperine treatment significantly decreased the up-regulated mRNA and protein levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) induced by DNFB in ear biopsy homogenates. Our findings suggest that aloperine greatly improves the DNFB-induced allergic contact dermatitis in mice. The therapeutic mechanism might be related to the reduction of TNF-alpha, IL-1 beta and IL-6 production induced by DNFB. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：147 / 152

页数：6