The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling

被引：380

作者：

Bi, Guozhi ^{[1
]}

Su, Min ^{[2
]}

Li, Nan ^{[2
]}

Liang, Yu ^{[2
]}

Dang, Song ^{[2
]}

Xu, Jiachao ^{[2
]}

Hu, Meijuan ^{[1
]}

Wang, Jizong ^{[4
]}

Zou, Minxia ^{[5
,6
]}

Deng, Yanan ^{[2
]}

Li, Qiyu ^{[2
]}

Huang, Shijia ^{[7
]}

Li, Jiejie ^{[5
,6
]}

Chai, Jijie ^{[7
,8
,9
]}

He, Kangmin ^{[2
,3
,10
]}

Chen, Yu-hang ^{[2
,3
,10
]}

Zhou, Jian-Min ^{[1
,10
,11
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[4] Peking Univ, Sch Adv Agr Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

[5] Beijing Normal Univ, Coll Life Sci, Beijing Key Lab Gene Resource & Mol Dev, Beijing 100875, Peoples R China

[6] Beijing Normal Univ, Coll Life Sci, Key Lab Cell Proliferat & Regulat, Minist Educ, Beijing 100875, Peoples R China

[7] Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Ctr Plant Biol,Sch Life Sci, Beijing 100084, Peoples R China

[8] Max Planck Inst Plant Breeding Res, Cologne, Germany

[9] Univ Cologne, Inst Biochem, Zuelpicher Str 47, D-50674 Cologne, Germany

[10] Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China

[11] Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China

来源：

CELL | 2021年 / 184卷 / 13期

基金：

中国国家自然科学基金;

关键词：

SINGLE-MOLECULE ANALYSIS; DISEASE RESISTANCE GENE; PLASMA-MEMBRANE; OXIDATIVE BURST; ARABIDOPSIS; EFFECTOR; DYNAMICS; PROTEIN; PSEUDOKINASE; RECOGNITION;

D O I：

10.1016/j.cell.2021.05.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in plants and animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane, The ZAR1 resistosome displayed ion channel activity in Xenopus oocytes in a manner dependent on a conserved acidic residue Glu11 situated in the channel pore. Pre-assembled ZAR1 resistosome was readily incorporated into planar lipid-bilayers and displayed calcium-permeable cation-selective channel activity. Furthermore, we show that activation of ZAR1 in the plant cell led to Glu11 -dependent Ca2+ influx, perturbation of subcellular structures, production of reactive oxygen species, and cell death. The results thus support that the ZAR1 resisto-some acts as a calcium-permeable cation channel to trigger immunity and cell death.

引用

页码：3528 / +

页数：26

共 76 条

[1] An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species [J].